Association Between Glycogen Synthese Kinase 3-beta Gene And Major Depressive Disorder Resting State Functional Magnetic Resonance Imaging

Objective:To examine the association between glycogen synthese kinase 3-beta gene fifth intron polymorphism and major depressive disorder resting state functional magnetic resonance imaging in order to further explore the pathogenesis of depression.Methods:Patients and Chinese Han health people were selected with strict standards. All subjects were collected general demographic information. The severity of depression was assessed by the 17 Hamilton (Hamilton Depression Scale, HAMD17); PCR and DNA sequencing analysis were used to detect the genotypes of GSK-3β.Subjects were grouped according to genotype; All subjects were scanned by 3.0T MRI scanner; Specialized data processing software was used to scan data pre-processing; Using REST software to calculate the value of each patient's ReHo and Alff value, software Spm5 was used between samples t test.Results:1. General demography data analysisThere were no significant difference in distributions of mean age, sex between different groups(P>0.05).2. Hardy-Weinberg equilibrium test resultsIn each sample GSK-3βrs6438552 genotype distribution consistent with Hardy-Weinberg genetic equilibrium (P>0.05), the samples which we chose were representative.3. Case and control group comparison between the various genotypes:(1) Case group compared with the control group (ReHo)①Patients who carrying C/C genotype compared with the control carrying C/C genotype found that in case group the left superior frontal gyrus's ReHo values higher than the control group, the right sub-occipital's ReHo value lower than the control group;②Patients who carrying C/T genotype compared with the control carrying C/T genotype had not found any special significance brain areas;③Patients who carrying T/T genotype compared with the control carrying T/T genotype found that in case group the left inferior frontal gyrus, right superior temporal gyrus and right superior frontal gyrus's ReHo value were higher than the control, and the left precentral gyrus's ReHo was lower than the control group.(2) Case group compared with the control group (Alff)①Patients who carrying C/C genotype compared with the control carrying C/C genotype found that in patient group the right cingulate gyrus's Alff value was higher than the control group;②Patient who carrying C/T genotype compared with the control carrying C/T genotype, results showed that in patient group the right temporal, right cingulate gyrus and left superior frontal gyrus AlfF's value were higher than the control group, and in the left superior temporal gyrus, left postcentral gyrus and other brain regions Alff value is lower than the control group;③We compared Alff value between patients who carrying T/T genotype and the control who carrying T/T genotype, results showed that in patient group the left precentral gyrus Alff value was lower than the control group.4. Based on the results of different genetic models grouped(1) The ReHo results showed that patients who carrying C/C genotype the right cerebellar anterior lobe ReHo significantly higher than C/T and T/T genotype combined group,control group who carrying C/C genotype the left caudate lobe of the cerebellum, left occipital lobe and left middle frontal gyrus ReHo value was higher than the C/T+T/T gene combined group.(2) Alff results showed that patients who carrying C/T+T/T genotype the left superior frontal gyrus and the left brain occipital Alff significantly higher than C/C genotype group, the control who carrying C/C genotype the left-brain occipital lobe Alff value was higher than the C/T+ T/T consolidated group;(3) ReHo and Alff results of the analysis show that patients who carrying T/T genetypes the left inferior frontal gyrus, left superior temporal gyrus and right middle frontal gyrus's ReHo and Alff value were higher than the C/C and C/T combined group.Conclusion:1. T/T genetype carriers may have a wider range of unusual activity on brain function.2. GSK-3βrs6438552 intronic polymorphism may related to the activities of resting-state brain function.3. GSK-3βgene may play a role in the occurrence and development of depressive disorders.