Expression And Clinical Significance Of Mesothelin And STAT3 In Pancreatic Carcinoma

Research backgroundIn resent years, the incidence of pancreatic cancer, the most malignant disease in digestive system, has appeared to increase gradually in all over the world. Mesothelin is a cell surface glycoprotein that is overexpressed in human pancreatic cancer. Although its value as a tumor marker for diagnosis and prognosis and as a preferred target of immunointervention has been evaluated, there is little information on the growth advantage of mesothelin on tumor cells. Resently, some forein researchers examined the effect of mesothelin on pancreatic cancer cell proliferation, cell cycle progression, expression of cell cycle regulatory proteins, and signal transduction pathways in two pancreatic cancer cell lines, MIA-MSLN (overexpressing mesothelin in MIA PaCa-2 cells) and BxPC-siMSLN (silencing mesothelin in BxPC-3 cells), and explored its role in pancreatic cancer pathogenesis.ObjectivesTo investigate the expression of mesothelin and STAT3 and the relationship between them and various clinical pathological characteristics in human pancreatic carcinoma.Methods(1) 45 cases of pancreatic cancer tissue sect serial sections for 6-8 sheets, respectively, for HE staining and immunohistochemical staining. HE sections observed under light microscope to observe the degree of differentiation.(2) The expression of mesothelin and STAT3 in tumor issues from 45 radically resected specimens of pancreatic carcinoma and 20 normal pancreatic tissues was detected by immunohistochemical staining.ResultsThe protein expression rate of mesothelin in pancreatic carcinoma was 80.0%, which were significantly higher than that in normal group.The Positive rate of mesothelin was closely related to degree of differentiation (P<0.05).The protein expression rate of STAT3 in pancreatic carcinoma was 77.8%, which were significantly higher than that in normal group. The Positive rate of cyclinE was closely related to the clinical stage and lymph node matastasis (P<0.05).There was a positive correlation between the positive rates of mesothelin and STAT3 (r=0.668,P<0.01).ConclusionsMesothelin and STAT3 were overexpressed in human pancreatic carcinoma.A positive correlation was found between the expression rates of mesothelin and STAT3. They might play an important role in the carcinogenesis and progress of pancreatic carcinoma.