Efficacy Of Sequential Therapy According To The Cell Cycle For The Adriamycin-induced Nephropathy Rats

Objective:To assess the therapeutic effects of three kinds of sequential therapy regimens according to the cell cycle on adriamycin-induced nephropathy(AIN) rats. Methods:Rat AIN model was established. SD rats were randomly divided into 6 groups: normal control group (n=6), AIN model group (n=8), Mp+Cs+CTX group (n=8),Mp+ Rap+CTX group (n=8), and Mp+LEF+CTX group (n=8). The rats in treatment groups were treated for 4 weeks. The level of 24 hour urinary protein, serum total protein (TP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST) were measured.Results:The level of 24 hour urinary protein in AIN model group was significantly higher than in the other four groups. The level of TP in AIN model group was significantly lower than in normal control group, Mp+Cs+CTX group and Mp+Rap +CTX group.The level of ALB was significant lower in AIN model group than in the other four groups, The level of 24 hour urinary protein, TP and ALB in Mp+Cs+ CTX group had more obvious changes than in Mp+Rap+CTX group and Mp+LEF+CTX group. Conclusions:According to the theory of cell cycle, three kinds of sequential combined regimens could decrease the excretion of urinary protein and increase the level of serum protein in adriamycin-induced nephropathy rats. The Mp+Cs+ CTX had the better therapeutic effects. Objective:Proteomics changes from the proteserum which isolated from the rat model of renal ischemia/reperfusion (I/R) injury are detected and investigated by the matrix-assisted UV laser desorption ionization time of flight mass sperctra (MALDI-TOF MS).Methods:After the establishment of rat renal I/R model, the serum samples which we selected respectively in 6,12,24 hours after reperfusion in each group were detected by MALDI-TOF MS analysis. And the peptide finger print which existed differences in each group were analyzed to identify. SPSS 13.0 software was used to the analysis the data. At the same time, we used Mascot Search to determine their nature in protein database.Result:1.The serum which was analyzed by IMAC-Cu bead was detected and had statistically significant peptide finger print in the m/z 2481 Da.2. The results obtained from peptide mass fingerprint (PMF) were analyzed by Mascot search program for protein identification. We identified it as rat fibrinogen fragment.Conclusion:Fibrinogen in kidney I/R injury plays an important role. Objective:To investigate the changes of fibrinogen in renal ischemia/reperfusion (I/R) injury rats using surface enhanced laser desorption ionizayion time of flight mass spectrometry(SELDI-TOF MS) technology.Methods:After establishing renal ischemia/reperfusion rat model, rats were divided into three groups:6 h,12 h,24 h reperfusion groups. The serum samples were chosen for the test. The peptide finger prints which existed differences in each group were analyzed by SELDI-TOF MS technology and rectified by Proteinchip Software3.1. Their characteristics were confirmed with MALDI-TOF/TOF MS by searching the protein database, and their contents were determined using ELISA in each group.Result:After the serum was detected by SELDI-TOF MS, the statistically significant peptide finger prints were identified in the m/z 2481 D. By searching the protein database, they were identified as rat fibrinogen fragments. The change of fibrinogen contents which was detected by ELISA was in accordance with the results detected by SELDI-TOF MS.Conclusion:Fibrinogen may play an important role in the renal I/R injury.