Association Between CD14 Gene Promoter Region Polymorphisms And Hsp Susceptibility

Objective To study the allele and genotype distribution of CD14 gene promoter region -159C/T,-260C/T polymorphisms in Chinese patients with HSP ,and to discuss the association between CD14 gene promoter region polymorphisms and HSP susceptibility.Objects and Methods1.Objects 144 cases of 2008 ~ 2009 in our hospital of the HSP children, are in line with, "Zhu Futang Practical Pediatrics," 7th edition of the diagnostic criteria . All children are Han Chinese, age 3 to 14 years old, the average (8.77±2.81) years, 77 patients were male and female 67 cases. Control group, 180 cases of physical examination in our hospital from the same period in the Chinese Han healthy children, aged 1 to 14 years with a mean (7.81±3.35) years, 104 patients were male and female 76 cases. Between the two groups by age, sex had no statistical difference.2.Methods Under the case-control study,CD14-159C/T and CD14-260C/T site polymorphisms in 144 patients with HSP and 180 healthy controls were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),the relationship between CD14-159C/T and CD14-260C/T site polymorphisms and the risk of HSP were analyzed.SPSS13.0 software was used to analyze the data.Results①HSP children alone in 144 patients with skin lesions in 25 cases, 60 cases of joint damage times, 74 cases of gastrointestinal damage times, 55 cases of kidney damage times; kidney damage in isolated hematuria in 1 case, simple 24 cases of proteinuria, hematuria with 30 cases of proteinuria, nephritic syndrome in 4 cases, 16 cases of nephrotic syndrome.②The distribution of CD14 gene -159C/T polymorphism was significantly diferent between GI involvement group,renal involvement group and control group;but the CD14 gene -260C/T polymorphism was significantly diferent between simple lesions group,renal involvement group and control group and the relative risk for different types of HSP in T allele carriers was higher than that in C allele carriers. Conclusion CD14 gene promoter region polymorphism is associated with HSP;and T allele of CD14 C(-260)T may be a risk factor for HSP.