Effects Of Qing Gan Fang On Liver Injury Induced By ConA And CCl₄

Objective:To investigate the effect of Qingganfang on the serum ALT,AST and pathologi-cal changes in ConA-induced and CCl4-induced hepatitis.Methods:The first part:46 ICR mice were randomly divided into four groups (A-Qingganfang group,B-Qingganfang together with polyporus polysaccharide (PPS) administered intraperitoneally into group,C-model group and D-normal group). Among the four groups, mice were fed with Qingganfang, Qingganfang together with PPS administered intraperitoneally into,distilled water, distilled water in order,twice a day,add up to 5 times.1 hour after medical treatments, mice of groups A,B,C was injected with ConA intravenously at a dose of 20 mg/kg body weight to induce hepatitis.Serum concentrations of TNF-a were determined using specific enzyme-linked immunosorbent assay kits after 2 hours, both IFN-y and IL-4 were determined after 8 hours in the same way; After 10 hours, the activities of liver enzymes aspartate trans aminase (AST) and alanine transaminase (ALT) in the serum were determined,and liver slice were Stained with hematoxylin and eosin (H&E).The second part:40 ICR mice were randomly divided into four groups (A-Qingganfang group,B-Qingganfang together with PPS administered intraperitoneally into group,C-model group and D-normal group). Among the four groups,mice were fed with Qingganfang, Qingganfang together with PPS administered intraperitoneally into, distilled water, distilled water in order,twice a day. The third day, mice of Groups A,B,C was injected with CC14 intraperitoneally to induce hepatitis,and Groups D was injected with edible oil in the same way.48 hours after the model was made,eyeballs were picked up for blood,then the activities of AST and ALT in the serum were determined. Results:①10h after injected in tail vein by ConA 20mg/kg,there were no remarkable differences of the values of ALT during A-Qingganfang group,B-Qingganfang together with PPS administered intraperitoneally into group,and D-normal blank group (141.87±71.94,106.36±34.31,85.17±23.08),but significantly slower than C-model group. (448.30±110.10)(p<0.01 & p<0.05); The level of AST in blank control group were slower than other three groups. The values of AST in Qingganfang together with PPS administered intraperitoneally into group were obviously slower than model group's.②HE dyeing of liver pathology demonstrated that nearby the model group central vein hepatocytes were seriously dropsical, the hepatocytes had hydropic degeneration and necrosis, simultaneously there were massive lymphocytes infiltration, spotted and focal necrosis appeared on the local scale,hepatic cord breaked.But there were no obvious differences in Qingganfang group and Qing gan fang together with PPS administered intraperitoneally into group③At 8h after ConA challenged, both A and B groups resulted in significant suppression of TNF-αIL-4,but activation of IFN-γ, than model group.④The level of TNF-αof both A and B groups(180.72±37.2,183.79±51.08)were slower than model group(292.82±63.96)(p<0.05),but higher than normal blank group (11.20±10.91) (p<0.01),at 2h after ConA challenged.⑤48h after CCl4 injected intraperitoneally,the level of ALT in serum were high-er than normal blank group(55.62±19.80)(p<0.01),pointing out that the model were made successful.The level of ALT in both A-Qingganfang group (97.38±72.64) and B-Qingganfang together with PPS administered intraperitoneally into group (117.67±35.42) were slower than model group(188.93±51.25)(p<0.05,p<0.01),but no differ-ences on AST.Conclusions:①Qingganfang has better function of reducing ALT,AST,presuming that it could prevent liver lesion by reducing ALT, AST and protect liver tissue. ②Qingganfang could result in significant suppression of TNF-αIL-4,but activation of IFN-γ,prompting that Qingganfang group were able to urge Th1/Th2 balance to drift to the Th1 direction, enhanced cellular immunity, in favor of clearing pathogen which invaded in vivo.③Qingganfang could decrease the level of ALT in the serum of mice with acute liver injury by perchlormethane(CCl4),and the mechanism is probably relating to in-hibiting the lipid peroxidation in histiocytes.