| Cordyceps sinensis and ganoderma lucidum as traditionary precious health remedy with medicine and food characteristics are applied generally since ancient times.Recorded by the documents cordyceps sinensis and ganoderma lucidum have effects of immunity enhancement,anti- tumor, anti-oxidant, stay caducity, blood sugar dropping,protection of liver and kidney and so on.Cordyceps sinensis and ganoderma lucidum polysaccharid as one of the most important chemical compositions and active compositions,whose prospect of utilization can not be ignored.At present, cordyceps sinensis and ganoderma lucidum preparation is directly fermented Cordyceps, Ganoderma lucidum fruiting body which are made of dried flour, except as mycelium and fruiting body composition with efficiency of things, contains a lot of impurities, more effective ingredients low, making cordyceps sinensis and ganoderma lucidum can not be fully effective active ingredients, medicinal value can not be fully realized. We use modern technology from the Cordyceps, Ganoderma lucidum polysaccharides extracted from fruiting bodies or complex, the active ingredients greatly enhanced.Moreover, in the market there is not any study and research on cordyceps sinensis and ganoderma lucidum polysaccharid compound which are mixed together as medicine. This test was made to study cordyceps sinensis polysaccharid and ganoderma lucidum polysaccharid compound table.which is made by according to given match.First of all, we studied the major composition of cordyceps sinensis polysaccharid and ganoderma lucidum polysaccharid and quality control research, Through the analysis of polysaccharide complex composition derived Cordyceps, Ganoderma lucidum polysaccharides mainly composed of complex polysaccharides, and contains amino acids and peptides, do not contain adenosine, phenols and tannins. By reducing sugar and total sugar content,the content of cordyceps sinensis polysaccharid is37.4%,and ganoderma lucidum polysaccharid is 33.5%。In vitro experiments it determined when volume ratio(μl:μl)of cordyceps sinensis polysaccharid and ganoderma lucidum polysaccharid is 3:1by lymphocyte transformation test,the result is the best.,and the inversion rate is obviously higher than single-use of cordyceps sinensis polysaccharid or ganoderma lucidum polysaccharid,at last this match was applied to prescription of dosage forms.In addition according to the result of analysis that cordyceps sinensis and ganoderma lucidum polysaccharid compound have better clearance activity of·OH and DPPH free radicals than single-use of cordyceps sinensis polysaccharid or ganoderma lucidum polysaccharid.Through the study we decided preparation technology of cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet: polysaccharid compound 10%,PVPP10%,salt lime79%,saponite0.5%,magnesium stearate0.5%;preparation condition: appropriate amount of 40%PEG6000 as adhesives,through 20 order sieve, drying on 60℃, repairing granulationes through 20 order sieve,and the addition method of disintegrant is in-terio. Finally, dissolution, weight variation and hardness of film study on the three batches of tablets prepared to validate, have reached an acceptable level.In order to verify the preparation of Cordyceps, Ganoderma lucidum polysaccharide complex tablet pharmacological activity,in this paper, observe the phagocytic function by peritoneal macrophage and the ability of carbon granular clearance;test the delayed-type hypersensitivity by inducing with dinitrofluorobenzene;test the ability of the spleen lymphocytes proliferation by MTT;the influence to mice′s humoral immunity were studie by hemolysin production level. The results showed that cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet can distinguishedly induce immunity enhancement, and has a dose-dependent.The levels of serum urea nitrogen, liver glycogen and the longest swimming time were determined before and after swimming test. The results showed that cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet has noticeable anti-fatigue effect . Cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet was evaluated on anti-hypoxic activity in several hypoxia mice models,including closed normobaric hypoxia and sodium nitrite or potassium cyanide toxicosis. The results showed that cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet has noticeable anti-hypoxic activity on mice. Through the above three validated in vivo activity of Cordyceps, Ganoderma lucidum polysaccharide complex tablet with improving immunity, anti-fatigue, anti-hypoxic activity.Finally, also in the cordyceps sinensis and ganoderma lucidum polysaccharide complex tablets was pharmacology and toxicology study, using the cordyceps sinensis and ganoderma lucidum polysaccharides in tablet concentration of the human body 100 times the amount of dose given to mice fed X days after the observation, this dose, control mice and mice treated group compared with the normal water intake, diet, mice of the state is good, smooth skins, no any adverse reaction, the mice were killed, dissected and visceral organs not found in any organ into lesions. The toxicology tests to prove tablets toxicity, oral administration of security. Developed quality standards for the cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet, by composition analysis of polysaccharide compounds de Rived tablet Cordyceps, Ganoderma lucidum polysaccharides in the same tablet as the main component of polysaccharide containing amino acids and peptides, do not contain adenosine, phenols and tannins. And disintegration, microbial limit, weight limit instant the degree of difference between checking, and the results are satisfactory. Determination of polysaccharide content in tablets by polysaccharide complex polysaccharide content of 3.25%.And then the methodology of assaying was inspected, Including stability, precision and sample recovery of inspection.The result showed that established analytical method have feasibility,good reproducibility and high accuracy.At length,studied the initial stability and accelerating stability respectively ,the result showed that after three groups sample product placed for 6 months, the detection of tablet characteristics, identification, examination had no significant change,which prove that the quality of the goods in the inspection phase stability.In influencing factor test,including high temperature test,high humid test and highlight exposure experiment,the result showed cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet had good stability from appearance to internal quality.In summary, this paper will be developed Cordyceps polysaccharides and Ganoderma lucidum polysaccharides made by a certain proportion of mixed compound, that is, Cordyceps, Ganoderma lucidum polysaccharide complex tablets, reasonable compatibility with drugs to produce an overall integrated effect,and the pharmacological experiments Cordyceps and Ganoderma active ingredients have complementary effects .As mentioned above, the research of pharmacology indicated cordyceps sinensis and ganoderma lucidum polysaccharid compound tablet had activities of immunity enhancement,anti-fatigue and anti-hypoxic, moreover cordyceps sinensis and ganoderma lucidum polysaccharid compound is safe and innocuity, possessing potentiality of developing good preparation. And this paper, Cordyceps sinensis, Ganoderma lucidum polysaccharides in-depth research, is bound for further study Chinese caterpillar fungus, Ganoderma lucidum pharmacological mechanism, improve the clinical efficacy, improvement of production process and quality control standards to provide the theoretical basis. |
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