The Expression Of Aurora-A Gene In Neuroblastoma And Its Relationship With Clinical Stage And Prognosis

ObjectiveNeuroblastoma is the most common malignant solid tumor in children which is derived from neural crest cell of sympathetic nervous system. As is studied, many genes were involved in the differentiation of neuroblastoma and the expression and regulation changed with the cell differentiation. Aurora kinases are a recently discovered family of kinases consisting of highly conserved serine/threonine protein kinases found to be involved in multiple mitotic events:regulation of spindle assembly checkpoint pathway, function of centrosomes and cytoskeleton, and cytokinesis. Aberrant expression of Aurora kinases may lead to cancer. It plays an important role in the genesis and development of many human malignant tumors. We aim to find a new and effective prognosis marker by detecting the expression of Aurora-A gene in neuroblastoma by real-time quantitative polymerase chain reaction(RQ-PCR) and investigating its relationship with clinical stage and prognosis.Material and method1,materialcollect 20 cases of frozen tissues and 15 cases of bone marrow of neuroblastoma of ShengJing Hospital and 10 cases of frozen tissues of ganglioneuroma as control group. The age is from 18 days to 9 years, less than 1 year 15 persons and more than 1 year 20 persons. All of the cases were diagnosed as neuroblastoma by pathology. According to Evans stage, all the cases are divided into low stage(stageⅠ:5 cases, stageⅡ:8cases) and high stage(stageⅢ:7 cases, stageⅣ:15cases).2,methodsextrction of mononuclearcell; extraction of RNA from mononuclearcell and tissues; reverse transcription reaction;real-time quantitative polymerase chain reaction(RQ-PCR)Results1,Aurora-A gene shows different exression levels in all the neuroblastoma patients and shows no expression in ganglioneuroma patients. There is obvious statistical difference(P<0.01);2,Aurora-A gene shows low expressions in low clinical stage(0.225±0.096), and shows high expressions in high clinical stage(0.659±0.079)(P<0.01);3,The expression levels of Aurora-A gene has relationship with ages. The patients less than 1 year low expressions (0.392±0.163) and the patients more than 1 year shows high expressions (0.578±0.237) (P<0.05);4,The expression levels of Aurora-A gene has negative correlation with survival time(P<0.01).Conclusion1,Aurora-A gene shows high expressions in malignant neublastoma, and it may be involved in the malignant generation;2,The patients with high expressions of Aurora-A gene has poor prognosis, and it can be a prognosis marker.