T Cell Immunoglobulin And Mucin Domain Protein(TIM3) In Multiple Sclerosis(MS) In Clinical Significance Of Expression

objectiveThis experiment measured multiple sclerosis and normal human T cells (CD3+), auxiliary T cells (CD4+), and regulate T cells (CD4+CD25+) cell subsets in Tim3 the percentage of positive difference, to explore the Tim3 in multiple sclerosis (MS) The role of the pathogenesis.MethodsThe 2008 to 2010 3 Department of Neurology in our hospital 7 males and 15 cases of hospitalized patients and 8 female as case group, aged 21-62 years old elect 15 disease-free immune system as healthy control group, age, gender and patient group match. case group,15 patients were diagnosed recurrent clinical MS patients are in line with McDonald 2001, proposed diagnostic criteria. And all patients within a month and no use of immunosuppressant corticosteroids. Admitted to hospital the next morning at 6 o'clock in MS patients taking the new admission peripheral venous blood (blood when all patients had not used corticosteroids), and normal peripheral blood collection. By flow cytometry.Results1. MS patients Tim3 in T cells (CD3+) and significantly lower in PBMCCD3+Tim3+cell subsets in PBMC in patients with multiple sclerosis in the percentage of 4.55%±0.53% was significantly lower than control group 6.31%± 0.59%, statistically significant differences (p<0.05). CD3+Tim3+cell subsets in multiple sclerosis T cells (CD3+) in the percentage of 7.2%±0.91% was significantly lower than the control group 10.42%±0.62% statistically significant difference (p <0.05).2. MS patients Tim3 the secondary T cell (CD4+) and significantly lower in PBMCCD4+Tim3+cell subsets in multiple sclerosis patients in the percentage of PBMC was 1.70%±0.30% was significantly lower than control group 3.03%±0.67%, statistically significant difference (p<0.05). CD4+Tim3+cell subsets in multiple sclerosis patients auxiliary T cells (CD4+) and the percentage of 4.3%±0.53% was significantly lower than the control group 8.7%±0.79%, statistically significant differences (p<0.05).3. MS patients Tim3 in the regulation of T cells (CD4+CD25+) and significantly lower in PBMCCD4+CD25+Tim3+cell subsets in multiple sclerosis patients in the percentage of PBMC was 0.63%±0.11% was significantly lower than the control group 1.45%±0.24%, a statistically significant difference (p<0.05). CD4+CD25+Tim3+cell subsets in regulating T cells in multiple sclerosis patients (CD4+CD25+) in the percentage of 11.59%±3.06% was significantly lower than the control group 24.13%±2.38%, statistically significant differences (p<0.05).ConclusionWith Tim3 Treg cell subsets in MS patients in the rate of reduction, suggesting that subsets of cells express Tim3 may have regulatory function of the sub-group, in the process of immune regulation play a positive role, it may be involved in the pathogenesis of the development of MS process, suggesting it as a marker of Treg, Treg reduce its regulatory function of the decrease of.