The Study On Protection Of Procyanidins Against UV-induced Oxidative Damage Of Lens Epithelial Cells

PurposeTo determine the protection of procyanidins against UVB-induced oxidative damage of lens epithelial cells with alkaline single cell gel electrophoresis and flow cytometry, in order to provide an experimental foundation for cataract clinical treatment.MethodThe lens epithelial cells (LECs) lines of generation 4 were divided randomly into 5 groups. As untreated group:normal cultured LECs; positive control group:normal cultured LECs+UVB; three treatrd groups:PC treated groups:LECs+PC+UVB; TAU treated groups:LECs+TAU+UVB; VC treated groups:LECs+VC+UVB. All groups of antioxidants were divided into 3 groups according to the final concentration from low to high (0.05mg/ml, 0.1mg/ml,0.2mg/ml). Each treated group was treated by antioxidants 2 hours before irradiated under UVB, respectively, then conventional cultivation. The LECs of treated groups and positive control groups were all irradiated at UVB-dose 15mJ/cm2. All of LECs in every group were collected after treatment an hour, respectively, and then were analyzed with alkaline single cell gel electrophoresis and flow cytometry.Results The differences of tail length, tail DNA% and tail moment between PC treated groups and positive control groups were statistically significant (P＜0.01), which is showed LECs treated with PC can resistant the damages of UVB. The differences of tail DNA%, tail moment in each PC treated groups and untreated groups were not significant (P>0.05). The apoptosis rates of 0.1mg/ml PC,0.2mg/ml PC and untreaded groups have no significant differences (P>0.05), which indicated that PC in those concentrations played more protective role against oxidative damage on LECs. The differences of tail length, tail DNA% and tail moment in various antioxidant treated groups were statistically significant (P＜0.01). The protective functions of PC treated groups were significantly higher than those of taurine treated groups and VC treated groups. The tail length, tail DNA%, tail moment and apoptosis rate of various antioxidant treated groups all decreased along with the concentration gradually increased.ConclusionExogenetic PC has protective effect to UVB-induced DNA damage. The protective effect of PC was obviously stronger than those of taurine and VC, and in a certain range of concentration, the protective effect of PC have the possitive relationship of dose-effect.