| PrefaceAtherosclerosis (AS) progress and plaque rupture is the cause of acute cardio-cerebral vascular events is mainly the result of ischemic stroke are important risk factors.Classic recipe Bu Yang Huan Wu decoction on the basis of addition and subtraction, developed Hua Zhuo Tong Mai powders, the application of modern medical methods to study the side effects of AS, through the intervention of high-fat diet animals to study the side on blood lipids, blood coagulation mechanism and the effects of eNOS expression, etc., and its prevention of AS, the role of a preliminary study.Atherosclerosis (AS) progress and plaque rupture is the cause of acute cardio-cerebral vascular events is mainly the result of ischemic stroke are important risk factors.So, the AS treatment on the prevention and treatment of cerebrovascular disease of great significance.Currently many Chinese medicine prevened AS, but still the main promoting blood circulation, classic recipe Bu Yang Huan Wu decoction with qi, promoting blood circulation, we conducted on the basis of this side addition and subtraction, developed to treat AS that Hua Zhuo Tong Mai powders, the application of modern medical methods to study the side effects of AS, through the intervention of high-fat diet animals to study the side on blood lipids, blood coagulation mechanism and the effects of eNOS expression, etc., and its prevention of AS, the role of a preliminary study. ObjectiveIn this study, by detecting blood lipids, blood coagulation mechanism changes, TXB2,6-Keto-PGF1α, ET, NO, and immunohistochemistry, microscopy pathology specimen handling, through the Hua Zhuo Tong Mai powders on AS treatment, to explore the side treatment of carotid atherosclerosis.Methods1.Building model:Adaptive feeding 1 week before the experiment, excluding abnormal diet after 1 week were randomly divided into four groups of 10:that Hua Zhuo Tong Mai powders group (A), Aspirin group (B), High-fat diet group (C), Control group (D), sub-cage rearing. D group, fed with normal feed, free drinking water, feeding to 12 weeks; the other three groups fed with high fat diet:A group fed with high fat diet, at the same time Hua Zhuo Tong Mai powders mixed into the feed, to 12 over the weekend, B group, fed with high fat diet, while adding the feed mixed with enteric-coated aspirin, to 12 over the weekend. C group to continue to give high fat diet feeding, did not add any medication, feeding to 12 weeks, experimental seventh day, B, C and A group were injected with Bovine Serum Albumin; D group was injected with normal saline.2.Biochemical examination method:Before the experiment and the end of the experiment, were treated with rabbit ear vein blood to measure blood lipids (TG, TC, LDL-C, HDL-C), four coagulation (PT, APTT, TT, FG),NO, ET,6-Keto-PGF1α, TXB2.3.The production of drawn and pathological specimens:End of the experiment, the application of anesthesia all animals were killed, trim left cervical artery, fixation, dehydration, embedding, slicing, for immunohistochemistry.ResultsHua Zhuo Tong Mai powders can effectively lipids, such as TC, TG, LDL-C, but increased HDL-C is not obvious, and can significantly prolong the clotting time of impact similar to aspirin, such as (PT, APTT, TT), and this side reduced fiber (Fg), also has a higher NO,6-K and reduced ET, TXB2 role, also improve the carotid artery eNOS expression.ConclusionsHua Zhuo Tong Mai powders has reduced blood lipids, anticoagulant, and reduced fibers, increased NO,6-K and reduced ET, TXB2, and can make increased expression of eNOS, so may protect arterial intima and slow the formation of atherosclerosis roles.
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