Extraction Of Liposoluble And Water-Soluble Components From Radix Et Rhizoma Salviae Miltiorrhizae With Microemulsion

Radix et Rhizoma Salviae Miltiorrhizae,a traditional Chinese medicinal materials with the function of promoting blood flow and removing blood stasis,has been generally applied to cure cardiovascular disease and cerebrovascular disease.The main active components are water-soluble salvianolic acids(Sals) and liposoluble tanshinones(Tans).Both sals and Tans have showed antiplatelet aggregative activity,anti-thromb,antioxidation, anti-artherosclerosis, anti-atherosclerosis and other effects. However,characterized by the structure of ortho-chinone or para-chinone, tanshinones are hydrophobic which leads to low bioavailability.Therefore, the salvia miltiorrhiza raw material extracting is usually alcohol extraction and then water extraction.Extracting two kinds of ingredients synchronously is time-consuming effort and does not favor raising production benefit.Microemulsion is made from oil phase, surfactant, cosurfactant and water phase. The oil phase and water phase can contain different physical and chemical properties of the the ingredients, especially different solubility of chemical ingredients, not only used as the carrier of hydrophobic drugs, but also as water-soluble drugs carrier, which can play a synergy between the components.The experiment take the microemulsion as the extraction solvent,ultrasonic extracting liposoluble and water-soluble ingredients from salvia miltiorrhiza, taking tanshinoneâ…¡A and danshensu as the indexes, determinating the content of indexes by HPLC and optimizing the microemulsion prescription by using ternary phase diagrams and uniform design.Eventually,Finalized ethyl acetate, Tween 80 and ethanol were the compositions of microemulsion components. And Tween 80:ethanol= 4:1, mixed surfactant:ethyl acetate= 6:4, the water weight ratio of optimized microemulsion is 70%, or ethyl acetate:Tween80: ethanol:water weight ratio is 12:14.4:3.6:70.The optimum microemulsion prescription chose the ratio of the solid to liquid, extraction time, extraction times for the factors, with orthogonal test method for further optimizing extraction conditions. With the total content of tanshinoneâ…¡A and Danshensu as an indicator to determine the optimum extraction process. The results showed that:the ratio of the solid to liquid, extraction time on the total content had a significant influence, extract times did not significantly affect the number of its. Accordingly, taking tanshinone â…¡A and Danshensu extraction content as the index, determine the optimum extraction:the ratio of the solid to liquid 1:20, extraction twice, extracting time 30 minutes.Recycling surfactant by heating up microemulsion and column chromatography. Through heating up microemulsion,surfactant reaching clouding point,settling down and separating from microemulsion,after that residual surfactant dosage is still large, about 50% of original content, tanshinoneâ…¡A and Danshensu suffered a minimal loss of content. By column chromatography, surfactant removed a large number of content,the content of tanshinoneâ…¡A and Danshensu suffered a corresponding loss, abour 68.9% and 57.7% of the original content respectively.