The Effection Of Hyperbaric Oxygen To Peripheral Blood CD34~+ Cells In Patients With Spinal Cord Injuries

Objective1.The study was designed to detect the absolute CD34 cell count in peripheral blood in control group and hyperbaric oxygen therapy group, that are patients with spinal cord injuries.2. To compare the different effects of the absolute CD34 cell count in peripheral blood between different times of hyperbaric oxygen intervention.3. To explore the possible mechanism of hyperbaric oxygen therapy in SCI. Methods1.The objects were the patients who visited our hospital during December 2009 to February 2010, and who were diagnosed as spinal cord injury. Screening for cases is accord to the inclusion criteria and exclusion criteria, and they are diagnosed with "flaccidity syndrome", according to syndrome differentiation.2. Randomized:the objects were randomly divided into two groups:The control group:routine treatment of spinal cord injury;Hyperbaric oxygen group:conventional therapy and hyperbaric oxygen treatment group.10 cases for the control group,11 cases for Hyperbaric oxygen group.3. Hyperbaric oxygen intervention:the group B were administer with 2. OATA pressure of oxygen for duration of 2h each day, persist a series of 20 days.4. The patients in A group were selected 2 tubes venous blood before and after the 5th day,10th day and 20th day during the routine treatment, each tube 2ml, which were fully anticoagulanted with heparin sodium. B group were selected 2 tubes venous blood before and immediately after the 5th day,10th day and 20th day during the treatment of HBO, each tube 2ml. The 2 tube were used for the detection of the absolute CD34 cell count and white cells(WBCs) in peripheral blood.5. The WBCs counts in peripheral blood:The WBCs in peripheral blood were tested dynamically by automated herpetology analyzer.6. The absolute CD34 cell count in peripheral blood:The number of peripheral blood CD34+cells were countted dynamically with ProCOUNT by flow cytometry7. Statistical analysis:All data points represent group mean±SEM,the date were analysed by Multivariate repeated measures analysis of variance, Independent-sample T Test and T'Test in SPSS16.0. Results1. After 20 sessions of hyperbaric oxygen treatment in hyperbaric oxygen group that contains 11patients with spinal cord injury, the absolute count of peripheral blood CD34+ cells was observed to be elevated from 3.93±0.82/μL to 14.82±2.40/μL,3.77 times compare to that before treatment. The number of WBCs was 6.61±1.40,6.44±1.07,6.41±1.29,6.40+1.29 respectivly before and the 5th day,10th day and 20th day during the treatment of HBO in Hyperbaric oxygen group, and was 7.05±1.32,6.79±1.06,6.92±1.29,7.02±1.02 respectivly in control group。2. The absolute count of peripheral blood CD34+ cells and the number of WBCs in hyperbaric oxygen group and control group were analysed by Multivariate repeated measures analysis of variance, found that hyperbaric oxygen therapy can elevate the absolute count of peripheral blood CD34+cells, while the role of white blood cells have not yet discovered. The estimated marginal means of peripheral blood CD34+cells in the two groups found two different variation. The absolute count of peripheral blood CD34+cells and the number of WBCs in different time point were analysed by comparison of two independent sample, which include Independent-sample T Test and T'Test. There were no statistically significant difference between hyperbaric oxygen group and control group with regard to the number of WBCs in different time point, and also to the absolute count of peripheral blood CD34+cells before treatment(P>0.05); The absolute count of peripheral blood CD34+ cells had statistical significance in the 5th,10th and 20th day(P<0.05).3. The average absolute CD34+cell count in different times of multiples compared with that of before the treatment of HBO in B group:The average absolute CD34 cell count in 5th compared to that of before the treatment of HBO is about 1.98 times, and 10th compared to that of before the treatment of HBO is about 3.14 times, and 20th is about 3.77 times.Conclusion1.Exposure to hyperbaric oxygen could mobilize bone morrow-derived stem cells in patients with spinal cord injury,and keep the absolute count of peripheral blood CD34+cells at a high level.2.In addition to the classical mechanism, hyperbaric oxygen may mobilize bone marrow stem cells so as to participation in a spinal cord injury.