Spleen Tongzhi Method Amyotrophic Lateral Sclerosis Motor Neuron Protection Mechanism Discussed

BackgroundAmyotrophic lateral sclerosis (amyotrophiclateral sclerosis, ALS) is a chronic progressive neurodegenerative disease, its etiology and pathogenesis and molecular genetic mechanisms may be related, There is no recognized effective modern medical treatment. The Chinese medicine in the treatment of this disease there are certain advantages and space, many physicians believe that the modern spleen and kidney deficiency is the basic pathogenesis of ALS, throughout the course of disease has always been. Therefore the use of Spleen and Kidney Diseases Tongzhi treatment of ALS, and discuss the possible molecular mechanisms and to evaluate the clinical efficacy of some significance.ObjectivesAnimal experiments by glutamate (glutamic acid, Glu) directly induced amyotrophic lateral sclerosis (ALS) spinal cord slice model, discussed splenorenal Tongzhi motor neurons selective for the protective effect; while simultaneously on the preliminary evaluation of splenorenal ALS patients before and after the relevant indicators of motor function.MethodsAnimal experiments by glutamate (Glu) direct induction of ALS spinal cord slice model, discussed splenorenal Tongzhi motor neurons selective for the protective effect; of spleen and kidney Tongzhi method for the protection of spinal cord motor neurons from Glu in nerve excitability injury and its mechanism. Methods A randomized controlled 5 groups SD rats were divided into control group, Glu model group, party group spleen, kidney side group, spleen and kidney side groups, placebo, Pi Gui Wan, complement in the Qi Decoction Gui Wan 2 weeks, from rat spinal cord lumbar enlargement Glu-induced production of ALS spinal cord slice model, adding the corresponding treatment group serum, cultured for 2 weeks, through the inverted microscope the morphology of motor neurons and LDH content of culture medium, SMI-32 labeled motor neurons immunohistochemical analysis was to compare the level of motor neuron damage.A retrospective study of the clinical diagnosis of ALS patients to this study, treatment of spleen and kidney Tongzhi 1 month, by ALSFRS rating scale on the main symptom scores before and after treatment compared to the initial method of spleen and kidney Tongzhi relevant indicators of motor function in patients with ALS effects.ResultThe experiments in organotypic spinal cord slice culture medium adding to the high concentration of Glu, through the inverted microscope, immunohistochemical staining, Hoechst 33258 immunofluorescent staining, tissue culture fluid content and LDH activity measured in tissue structure such as the spinal cord was found that Glu model of spinal cord ventral horn of SMI-32 positive of a reduction in the number of motor neurons, spinal cord cells on activity decreased and the LDH content in culture medium increased (P<0.05), the difference was statistically significant, while the intermediate dorsal horn neuron damage relatively light (P> 0.05), the difference was not statistically significant; the treatment of spinal cord ventral horn neurons in dorsal horn of the decrease in the number of spinal cord cells on activity decreased and the LDH content in culture medium increased (P> 0.05), the difference was not statistical significance.ALS patients compared before and after the ALSFRS total score (P<0.05), the difference was significant; ALS patients after the speech, salivation, swallowing, independent eating, turning in bed, walking, breathing and other symptoms scores compared with before treatment (P<0.05), the difference was significant; ALS patients after the treatment writing, dressing, and other symptoms CASTLE points compared with before treatment (P> 0.05), the difference was not statistically significant. ConclusionAnimal experiments by direct application of glutamate-induced spinal cord slice model of ALS preliminary results show that the cells, the synaptic cleft produced high concentrations of Glu excitotoxicity, causing damage to motor neurons, so that a 32-SMI-positive a movement reduce the number of neurons, spinal cord cells on activity decreased; the spleen and kidney Tongzhi method for the cell, the synapse between the high concentration of Glu caused by motor neurons has a protective effect that can delay the progress of the disease.Rating Scale through ALSFRS ALS patients before and after treatment comparison of the major symptom score, after 1 month of the initial prompt, the patient's speech, salivation, swallowing, independent eating, the bed stood up, walking and other symptoms of respiratory function was improved, indicating that spleen and kidney Tongzhi method can improve the symptoms of ALS patients, delaying the progress of the disease.