Protective Effect Of Pyrrolidine Dithiocarbamate Pretreatment On Myocardium Against LPS-induced Injury In Rats

Objective To investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) pretreatment on myocardium against endotoxin-induced injury in rats.Methods Seventy-two Wistar rats of both sexes weighing 200-250 g were randomly divided into 3 groups (n=24 each):groupⅠnormal control (group C); groupⅡLPS and groupⅢPDTC. Endotoxemia was induced by intraperitoneal lipopolysaccharide (LPS) 8 mg/kg (5mg/ml) in groupⅡandⅢ. In groupⅢPDTC 120 mg/kg (60mg/ml) was injected iv at 30 min before IP LPS. Venous blood samples were collected at 1 h (T1),3 h (T2),6 h (T3), and 12 h (T4) after LPS administration for determination of serum cTnT concentration by ELISA. Six animals were killed at each time point after blood sample collection. Myocardial specimens were obtained for microscopic examination and determaintion of TLR4 and NF-κB p65 expression by immuno-histochemistry.Results Intraperitoneal LPS significantly increased serum cTnT concentration and myocardial TLR4 and NF-κB p65 expression in groupⅡ. PDTC pretreatment significantly attenuated LPS-induced increase in serum cTnT concentration and myocardial TLR4 and NF-κB p65 expression. Light microscope examination showed that the myocardial damage induced by LPS was also ameliorated by pretreatment with PDTC.Conclusion LPS induced injury on myocardial tissues in rats. Pyrrolidine dithiocarbamate pretreatment can protect myocardium against LPS-induced injury through inhibition of activation of myocardial NF-κB p65 and TLR4.