| Objective:1 Observe the intestinal structure and barrier function of SD rats treated with 5-Fu intraperitoneal injection,compare the effect of different scheme of 5-Fu adminis-tration on the intestinal barrier.2 Investigate the role of Lactobacillus in the protection of the intestinal barrier function in such situation.Methods:48 SD rats were divided into six groups,each group with 8,they were control group of low-dose(A),5-Fu in low dose(B), Lactobacillus with low dose 5-Fu(C), control group of high-dose(D),5-Fu in high dose (E), Lactobacillus with high dose 5-Fu(F).Low dose groups daily injection 5-Fu 50mg/kg intraperitoneal D1-5,high dose groups injection 5-Fu 250mg/kg intraperitoneal on the first day, control groups inject saline instead. Lactobacillus were given to group C,group F orally D1-7,other groups were given saline instead.The weight and diarrhea were data everyday.On the eighth day killed all rats in each group, to investigate the pathological changes and ultrastructure of intestinal mucosa, survey and evaluate endotoxin in blood, protein content in the intestinal mucosa and the expression of NF-κB.Results:1. WeightRats in group A and group D body weight increased daily while others loosed body weight continuously.There are three rats were dead respectively in group B,C,E. On the weight lose of rats in group B,C,E,F was significantly compared with control groups,P＜0.05.On the eighth day,weight of rats in group B,C,E,F dropped further,the weight loss of rats in group F less than other three groups,P＜0.05. 2. DiarrheaOn the 72th hour, Rats in group E appeared the most serious diarrhea. The situation in rats of group B was worse than that in group C,group E worse group F,group E was worse than that in group B, P＜0.01. On the eighth day,Rats in group B,C,E suffered from diarrhea. The situation in rats of group B was worse than that in group C,group E worse group F,group E worse group B,The situation in group B was worst,P＜0.01.3. Endotoxin in bloodOn the eighth day, Rats in group B,C,E,F had wores endotoxemia than control groups. Group B was worse than that in group C, group E worse group F,P＜0.01; Group B was worse than that in group E, group C worse group F,P＜0.01.4. Pathological scoreOn the eighth day, the pathological score in rats of group B was higher than that in group C,group E higher than group F; group B was higher than that in group E,Group B got the highest score and the intestinal mucosa was damaged the most severely,P<0.01.5. Intestinal mucosa ultrastructureOn the eighth day, Rats in control group had normal ultrastructure, while others had abnormal ultrastructure (such as microvillus, tight junction, and intercellular space) varying degrees changes. Group E was worse than that in group F,group C was worse than both groups above-mentioned; Group B was worst,P＜0.01.6. The expression of NF-κBOn the eighth day, the expression of NF-κB in rats of group E was higher than that in group F,group B higher than group E; group C was higher than that in group F, P＜0.05.Group B higher than group C, P＞0.05.7. Protein content in the intestinal mucosaOn the eighth day, the protein content in the intestinal mucosa in rats group B,C,E,F were lower than the control groups. Group B was lower than that in group C, group E lower group F,P＜0.01; Group B was worse than that in group E, group C worse group F,P＜0.05.Conclusions:1 Rats treated with the injection of 5-Fu intraperitoneal can successfully found the model of intestinal barrier dysfunction, due to endotoxemia brought about by inflammatory reaction (owing to activation of NF-κB), tight junction damage, protein content in the intestinal mucosa losing. 2 The administration scheme of 5-Fu injection intraperitoneal as 50mg/kg for 5 days caused much severe damage to the intestinal mucosa than the scheme of 250mg/kg at once. This result may be related with amplification effect of inflammatory reaction,3 Given Lactobacillus orally can protect the intestinal mucosa in the rats suffered from 5-Fu injection.4 Lactobacillus mitigate cell inflammatory reaction, tight junction damage, protein content in the intestinal mucosa losing. |
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