Influence Of Qiyiyiganjian On Expressions Of Survivin,Caspase-3 And Cyclin D1 On H22 Cells-bearing Mice

Objective:Hepatocellular carcinoma(HCC) is one of the common types of malignant tumors with poor prognosis,metastasis and high fatality rate.Traditional Chinese Medicine(TCM) has an important position in treat-ment of hepatocellular.TCM not only enhances the body's immune function, but also mobilizes the body's own cancer-fighting ability, thereforce it can inhibite tumor proliferation and metastasis and prolong survival.TCM has become the key to improve the clinical efficacy in the comprehensive treatment of HCC, and the mechanism of traditional Chinese medicine has the features of multi-site, multi-link, multi-target action to antitumor.Occurrence and development of Hepatocellular carcinoma is the results of a multi-gene mutation and multi-link out of control. Imbalance of apoptosis inhibition and cell cycle regulation is the important mechanism. In order to further explore the mechanism of QiYiYiGanJian(QYYGJ) on apoptosis and proliferation inhibition in mice, we explore the antitumor effect function and protect immune organ of QYYGJ on H22, at the same time, we explore the expressions of Survivin,Caspase-3 which are the key proteases of apoptosis and Cyclin D1 which is the key regulatory protein in G1 in the H22 tumor-bearing mice, all those can provide new ideas for the prevention and treatment of primary liver cancer.Methods:After injecting H22 hepatoma cell into their right axila,30 mice were randomly and equally divided into three groups:control group,treatment group and 5-Fu group. After 24h of postoperation, Control group received treatment of gem free saline,treatment group treated by QiYiyiganjian and 5-Fu group treated by 5-Fu.15 days later inhibiting rate was caculated, their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. Survivin Caspase-3 and Cyclin D1 were examined by using immunohistochemistry straining method in the tumor tissue.Results:1. The tumor inhibiting rate of QYYGJ group was 37.28%, and 5-FU group was 50.48%;2.The influence of QYYGJ on the thymus and spleen indices:Compared to 5-Fu group, treatment group could inhance the thymus and spleen indices significantly (P<0.01);The difference between control group and treatment group is significant in thymus indices (P<0.01), but is not significant in spleen indices; The thymus and spleen indices are low in 5-Fu group, the difference between control group and 5-Fu group is significant in spleen indices (P<0.05), but is not significant in thymus indices;3.The results of immunohistochemisty:Expressions of Survivin in H22 cells are positive in three groups. the expressions of Survivin in QYYGJ group and 5-Fu group are decreased and apparently different from that of control group (P<0.05, P<0.01); Expressions of Caspase-3 in H22 are positive in three groups. The expression of Caspase-3 in QYYGJ group and 5-Fu group are increased and apparently different from that of control group (P<0.01); Expressions of Cyclin Dl in H22 cells are positive in three groups. the expression of Cyclin Dl in QYYGJ group and 5-Fu group are decreased and apparently different from that of control group (P<0.01).Conclusions:1.QYYGJ has apparently inhibiting effects on H22 cells-bearing Mice;2.QYYGJ can inhance the weights of thymus and spleen in H22 cells-bearing Mice,this indicates that QYYGJ has the function of protecting immune organs; 3.QYYGJ can decrease the expression of Survivin and increase the expression of Caspase-3 in H22 cells-bearing Mice;4.QYYGJ can decrease the expression of Cyclin D1 in H22 cells-bearing Mice...