| Objective: To study the placental expression of MRP1,MRP2 in third trimester of pregnancy and their relationship with total bile acid, which helps doing further study on the effects of the three proteins on intrahepatic cholestasis of pregnancy.Methods: Pick up 30 ICP cases, who have been diagnosed during Mar.2008 and Oct.2009, as the ICP group and 30 normal pregnant women at the same period as the control group. The expression of placental MRP1,MRP2 are detected in immunehistochemistry, the maternal and umbilical total bile acid levels are detected as well.Results: (1) Both MRP1 and MRP2 express in placenta in both groups. (2) The expression level of MRP1 in placenta in ICP group is higher than control group(2.57±1.43 VS 1.67±0.85,P<0.01). (3) The expression level of MRP2 in placenta in ICP group is lower than control group(1.47±0.82 VS 2.43±1.20,P<0.01). Moreover, there is a negative correlation of the MRP2 expression level and the umbilical total bile acid level in ICP group(R=-0.72,P<0.01)(.4) Both maternal and umbilical total bile acid levels in ICP group are higher than control group(34.61±23.25 VS 2.04±1.48,13.39±9.06 VS 3.45±1.86,P<0.01). (5) There is a positive correlation of maternal and umbilical total bile acid in ICP group(R=0.967,P<0.01).Conclusion: Both MRP1 and MRP2 express in placenta in third trimester of pregnancy. With the increasing level of TBA, the expression of MRP1 rises while MRP2 falls, which mean the two proteins participate in the transporting of bile acids. The varieties of their capacities of transporting may causes the cholestasis in fetus. there is a negative correlation of the MRP2 expression level and the umbilical total bile acid level in ICP group. It illustrates that MRP2 may be plays an important role in bile acids transporting in placenta. The maternal and umbilical total bile acid levels are higher in ICP, and there is a positive correlation of them. Detecting the maternal TBA level can help understanding the cholestasis of fetus.
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