Effect Of RhIGF-1 On The Proliferation,BMP-2 And Cbfa1 Genes Expression In Cultured Rat Osteoblasts

Objective To study the effect of rhIGF-1 on the proliferation, BMP-2 and Cbfalgene expression in rat osteoblasts in vitro.Methods:1,Rat osteoblasts in primary culture and their identification.2,Cultured rat osteoblasts were treated with rhIGF-1 in different concentrations(0,10,50,100 ng/ml), cell proliferation was assessed by MTT colorimetric assay.3,Cultured rat osteoblasts were treated with rhIGF-1 in different concentrations(0,10,50,100 ng/ml), the expression of BMP-2,Cbfal genes in rat osteoblasts were examined by semiquantitive RT-PCR.4,Statistical analysis:All statistical analysis was performed with Windows SPSS 13.0, date are presented X±S. Homogeneity of variance between groups compared with the LSD-t test; variance missing, between the two groups compared with DunnettT3 test. Differences were considered significant at a value of P<0.05.Results:1,Primary cultured cells were identified as osteoblasts.2,Cell proliferation was assessed by OD that though MTT. Result shows that proliferation of cultured rat osteoblasts with rise of concentrations of rhIGF-1,and in a does-dependent manner.Control group and experimental group, the experimental group between the two groups, were statistically difference(P<0.05).3,Semi-quantative RT-PCR examination revealed that BMP-2 and Cbfal gene expression was increased with rise of concentrations of rhIGF-1,optical density increased significant, and in a does-dependent manner.Control group and experimental group, the experimental group between the two groups, were statistically significant difference (P<0.01).Conclusions:1,rhIGF-1 can promotes proliferation in cultured rat osteoblasts.2,rhIGF-1 increase the expression of BMP-2 and Cbfal gene.3,rhIGF-1 induces the proliferation,differentiation and maturity of osteoblasts probably by increasing the expression of BMP-2 and Cbfal gene.