Study On The Mechanism Of Cytotoxicity Induced By Indium Sulfate And Vc Rival Effect

Objective: Indium is a metal in the nature, which is widely used in the industry, and the workers engaged in the indium industry are increasing. The indium which is absorbed into the blood is rapidly transported to the soft tissue and the bone. The colloid indium is sent to the reticulo-endothelium system of the liver and spleen after it is swallowed by the leucocyte. The indium primarily accumulates in the bones of the body. When hypodermic injection, the most of the indium stores up in the skin and muscle. When the abdominal injection, the indium stores up in the mesentery and the liver, then it shifts to the spleen, the kidney , the skeleton and so on. The metal indium is non-toxic in fact. However the soluble indium compound is poisonous. The indium sulfate is a representative of the soluble indium compound.The aim of the research is to examine the effect of indium sulfate on cell viability and oxidative stress injury, to explore the toxic mechanism and the rivalry of vitamin C. In the study, we discuss the mechanism of the cytotoxicity and to find the effective antagonist. And it can provide the laboratory data for the prevention of the environmental exposure in the future.Methods: The research is mainly divided into three parts. First, the viability of V79 cells is measured by MTT method and the dose-effect relation could be decided. According to the result, we can make the dosage range of the indium sulfate for the rest of the experiment. Second, the purpose is to observe the influence of indium sulfate to the content of total protein and malonic dialdehyde(MDA), the cell apoptosis rate, the cell cycle distribution and the vigor of the superoxide dismutase(SOD). Third, we discuss the influence of indium sulfate to the cell apoptosis rate, the energy of SOD and the MDA content when we mix the vitamin C to the cell culture food.Results: As showed in the MTT assay, V79 cell proliferation was inhibited obviously by indium sulfate (treated 2hours, from the concentration of 8 mmol/L or treated 24hours, from the concentration of 2mmol/L), and concentration–dependent relationship and time-dependent relationship were show(P<0.05). Under the function of indium sulfate, the mitotic cycle distribution was changed and the cell apoptosis rate was increased. The cell content in G1 and G2 / M phase were reduced, but the cell content in S-phase was increased . The SOD activities were reduced, but the levels of MDA were raised with the increase of the indium sulfate concentration. The statistic difference was significant (P<0.05). But the total cell protein content did not change. And the vitamin C can rival the change of SOD and MDA. The statistic difference was significant (P<0.05).Conclusion: The indium sulfate can inhibit the V79 cell proliferation with low concentration in short time. The indium sulfate changes the distribution of cell cycle and increases the cell apoptosis rate. The indium sulfate weakens the vigor of SOD and increases the content of MDA. But the vitamin C can decrease the apoptosis rate in low concentration. The vitamin C rival the oxidative stress injury induced by indium sulfate. From the above, it indicates that the V79 cell is in an active state of oxidative stress. Subsequently the lipid peroxidation changes the distribution of cell cycle and increases the cell apoptosis rate.