Preparation Of Emulsion Polymerization Monolith And Its Application For The Analysis Of Drug In Human Plasma

High performance liquid chromatography plays an important role in pharmaceutical analysis. Column is the heart of the HPLC, so the bulking agent of the column has an influence on the efficiency of the separation. Unfortunately, due to the complexity of biological fluids, the direct injection of these samples is not compatible with most chromatographic systems. A sample preparation step is necessary to carry out. In addition, because of the low concentration of medicines in biological samples, enrichment is needed for trace analysis. On-line solid phase extraction is utilized to overcome these problems.In this paper, the development of monolithic column was reviewed. The preparation and modification of polymeric monolithic column were also introduced. Moreover, the application of the monolithic column for the pretreatment of biological samples on HPLC was described.Furthermore, a novel emulsion polymerization monolithic column was prepared using glycidyl methacrylate as monomer. Using this monolith as SPE material, m-nisoldipine in human plasma could be on-line enriched and meanwhile the protein could be eluted. Moreover, twelve-hour pharmacokinetic profile of m-nisoldipine in mouse after oral administration was investigated. On-line column switching devices combined with such kind of monolith could be applied to on-line remove matrix compounds in biological fluid and simultaneous enrichment of small molecule medicine. Exploration of the material is expected to solve the traditional sample pretreatment problems such as extensive manipulations, large sample volume, low extraction efficient and environment contamination.At last, a new emulsion polymerization molecular imprinted polymer of nifedipine was synthesized using methacrylic acid as the monomer. In addition, a solid-phase extraction column packed with nifedipine-imprinted polymers was used to enrich the nifedipine. Results indicated that it provided a simple and fast method for assaying nifedipine in plasma sample and this method could be applied for monitoring of nifedipine.