The Effects Of Formoterol And ICI118551 On Rat Ostoeclast In Vitro

【Objectives】: To evaluate the effects of Formoterol (β2-adrenergic receptor-specific agonist) and ICI118551 (β2-adrenergic receptor-specific antagonist) on rat matured ostoeclast cells in vitro.【Methods】: Mature OC were isolated directly from neonatal 24-hour-SD rat long bones. The OC was treated with different concentrations of Formoterol and ICI118551 from 10^-5mol/L～10^-9mol/L respectively. Then the OC was stained with tartrate-resistant acid phosphatase (TRAP) to observe cellular morphology. The bone slices were stained with toluidine blue to count the number of the bone resorption pits and analyse the bone resorption pits by Image-Pro Plus 6.0.【Results】: The number and area of the bone resorption pits increased in different concentrations of Formoterol treatment compared that in control treatment after osteoclasts and bone slices co-cultured for 6 days. Furthermore, the number and area of the bone resorption pits decreased with the dose of ICI118551increased gradually.【Conclusions】: Theβ2-adrenergic receptor-specific agonist could promote the bone absorbion of OC in vitro. Theβ2-adrenergic receptor-specific antagonist inhibits the bone resorption of OC in a dose-dependent manner.