| It took only a few years from the discovery of Cis-platinum having antitumor characteristic to clinical application, but there's little coverage of ruthenium (II) polypyridyl complexes used as anti-cancer drug since they have been claimed that they having anticancer activity in the early 1980s, it attempts to be done in this area. Octahedral ruthenium (II) polypyridyl complexes have affluent photochemical and photophysical information, it is very suitable for studies on the molecular recognition and interaction between ruthenium (II) polypyridyl complexes and DNA.The current methods of studing interaction between ruthenium (II) polypyridyl complexes and DNA are mainly spectroscopy method, NMR spectroscopy method and fluid dynamics method. In recent years, due to operational simplicity and high sensitivity, resonance light scattering (RLS) has aroused extensive attention and successfully been applied in all fields of analytical chemistry research. It has been rarely reported on studies of interaction of ruthenium (II) polypyridyl complexes with DNA using RLS method. In this paper, we choose three kinds of non-water-soluble ruthenium (II) polypyridyl complexes to further study their interaction patterns with DNA by RLS method and a number of other techniques, which can provide some guiding value in filterring anticancer drugs. Meanwhile, compared to other highly toxic probe, such as ethidium bromide etc., ruthenium (II) polypyridyl complexes have great advantage because of its none toxic side effects, therefore, we choose three kinds of ruthenium (II) polypyridyl complexes as RLS probe to quantitatively detect DNA. These methods are simple, rapid, high sensitive and with good reproducibility, and the results are satisfactory.This paper has four chapters.In chapter one, we briefly introduce the chemical composition of DNA, the studing methods of interaction between Ru (II) polypyridyl complex and DNA, and current methods of quantitative analysis and determination of DNA. We focused on the principle of resonance light scattering methods and the foundation of quantitative analysis.In chapter two, we synthesized Ru (II) polypyridyl complexes [Ru(phen)2(bpip)] (ClO4)2·H2O and studied the principle and application of its interaction with DNA by resonance light scattering methods.We established a new method to determinate ctDNA using [Ru(phen)2(bpip)]2+as a RLS probe. Under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of ctDNA, over the range of 0.0-1.296mg/L. Its Linear formula was△IRLS= 283.14C + 2.26 (mg/L), the correlation coefficient was 0.9983 and the limits of determination was 5.7ng/mL.In chapter three, we synthesized Ru (II) polypyridyl complexes[Ru(bpy)2(btip)] (ClO4)2·2H2O and studied its interaction principle and application with ctDNA by resonance light scattering methods.We established a new method to determinate ctDNA using [Ru(bpy)2(btip)]2+as a RLS probe. Under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of ctDNA, over the range of 0.0-1.89mg/L. Its Linear formula was IRLS=242.01C +135.5(mg /L), the correlation coefficient was 0.9967 and the limits of determination was 10.9ng/mL.In chapter four, we synthesized Ru (II) polypyridyl complexes [Ru(bpy)2(pmip)] (ClO4)2·2H2O and studied its interaction principle and application with ctDNA by resonance light scattering methods.We established a new method to determinate ctDNA using [Ru(bpy)2(pmip)]2+as a RLS probe. Under the optimum conditions, the enhanced RLS intensity was proportional to the concentration of ctDNA, over the range of 0.0-2.4mg/L. Its Linear formula was△IRLS= 144.09C+7.44 (mg /L), the correlation coefficient was 0.9985 and the limits of determination was 6.04ng/mL. |
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