The Influence Study And Preliminary Mechanism Of (-)-epigallocatechin Gallate On Invasion And Metastasis Of Human Ovarian Cancer Cells

Ovarian cancer, as a class of common cancers of the female and the fifth high incidence cancer, is a serious threat to women's health and more than 80% of patients were died from the metastasis of cancer at present. Therefore, the prevention of malignant invasion and metastasis of cancer cells is an effective treatment against ovarian cancer.Inflammation has been considered to be an important factor in the development and progression of tumors. Studies have shown that local existing inflammation, induced tumors by DNA damage, apoptosis and so on, could raise a lot of immune cells invasion, thus form the microenvironment to promote the development of tumor.Inflammation has been regarded as an important component of carcinogenesis and cancer progression. It is increasingly clear that the inflammatory tumor environment promote the neoplasia process, cancer cell proliferation, survival and migration.Epigallocatechin gallate EGCG as the main component of green tea, have a wider anti-tumor and anti-inflammatory activity. The anti-tumor mechanism of EGCG has not been understood systematically and clearly, and remains to be further studied. Therefore, we selected the low-metastatic ovarian carcinoma cell lines HO-8910 and the highly-metastatic ovarian carcinoma cell lines HO-8910PM differentiated from HO-8910 as materials. We treated cells with different doses of EGCG, and selected 50μM EGCG which inhibited the growth of cancer cell significantly as a final working concentration.Then, we pretreated the ovarian cancer cells with 50μM EGCG, and detected the phenotypic changes in the control and experimental group of the two ovarian cancer cells by the experiment of tumor cell colony formation in soft agar, wound healing and matrigel infection assays. The results proved that HO-8910PM showed greater proliferation and transfer activity compared with HO-8910; 50μM EGCG could significantly inhibit the growth capacity of non-anchor, migration, invasion and metastasis of two ovarian cancer cells; the effect on the high transfer cell lines HO-8910PM was particularly significant.Finally, we pretreated the ovarian cancer cells with 50μM EGCG again, detected the expression changes of gene IL-8, TNF-αand gene MMP9, VEGF by real-time quantitative PCR on mRNA level, and detected the level of inflammatory cytokine IL-8 and TNF-αby ELISA. The results showed that 50μM EGCG reduced the expression of inflammatory gene IL-8 and TNF-αin highly-metastatic line HO-8910PM significantly, while the down regulation effect on low-metastatic line HO-8910 was not significant; 50μM EGCG had no influence on the expression of gene MMP9 and VEGF.In summary, we believed that EGCG could reduce the growth capacity of cancer cells, while low doses of EGCG could reduce the biological characteristics of invasion and metastasis of malignant tumors contrarily. This effect of EGCG was likely to slow down the inflammatory damage to further weak the malignant phenotype of tumor cells by intervening the expression of inflammatory factor IL-8 and TNF-α. Concerning the relevance of anti-tumor and anti-inflammatory activity of EGCG will further explore the application of its new target for anti-tumor innovative ideas.