Correlation Of ATG16L1, TLR4 And TLR2 Gene Polymorphisms With Inflammatory Bowel Disease In Zhuang Population In Guangxi Province

Objective To study the relationship between ATG16L1 gene Thr300Ala,TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp polymorphisms and the susceptibility to inflammatory bowel disease(IBD) in zhuang population in Guangxi Province.Methods Intestinal tissue samples of seventy Zhuang and seventy-six Han unrelated IBD patients and eighty Zhuang and eighty-four Han unrelated healthy people as controls,were collected in Guangxi region from Feb, 2007 to Oct, 2010.Genomic DNA was extracted from the intestinal tissue by phenol chloroform methodology.ATG16L1 gene Thr300Ala,TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp were amplified by polymerase chain reaction (PCR),the PCR products of ATG16L1 gene Thr300Ala were sequenced.The sequenced results were compared with the normal sequence in Genbank.TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),some PCR products of TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp were sequenced .Results No significant differences were noted in the ATG16L1 gene Thr300Ala genotype and allele frequency among the IBD patients with Crohn's disease, ulcerative colitis and the healthy controls in Zhuang and Han population in Guangxi Province (P > 0.05).The mutation genotypes of the TLR4 gene Asp299Gly,Thr399 Ile and TLR2 gene Arg677Trp,Arg753Gln were not found among the population.Conclusions The ATG16L1 gene Thr300Ala, TLR4 gene Asp299Gly, Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp polymorphisms are not associated with IBD in Zhuang population of Guangxi Province.