| Objective: To investigate the regulation of MMP-2mRNA and TIMP-2mRNA expression by disintergrin kistrin in lens epithelial cells and explore the mechanisms responsible for the regulation.Methord: 12 New Zealand white rabbits were randomly devided into control group and experimental group,6cases each group.Extracapsular lens extraction was performed on right eye of each rabbit to establish the model of posterior capsular opacification .0.2ml 80μg·L-1 kistrin was injected into the capsular bag of lens in experimental group and the normal saline 0. 2 mL in the control group just after the operation. 6 left eyes were choosed randomly as blank group. All eyes were observed by slit-lamp microscopy at first day,second day, third day, 7th day and 14th day. 14 days after the operation ,take out the subcapsule and lens epithelial cells for real-time RT-PCR.to exam the expression of MMP-2mRNA and TIMP-2mRNA.Result: MMP-2mRNA and TIMP-2mRNA could be detected in normal Rabitt lens epithelial cells,and the abundance were 0.127±0.039,2.485±0.507 respectively. The abundance of MMP-2mRNA was 3.785±0.934and that of TIMP-2mRNA was 19.903±10.499 on 14th day after surgery.The expression of MMP-2mRNA and TIMP-2mRNA increased significantly compared with the blank group (p<0.05). In the experimental group, the abundance of MMP-2mRNA was 0.401±0.253,which decreased obviously compared with the control group(p<0.05).There were no difference between the experimental and the blank group(p>0.05). The abundance of TIMP-2mRNA was 16.119±3.773 in the experimental group and there were no difference between the experimental and control group in the expression of TIMP-2mRNA(p>0.05),but it increased obviously compared with the blank group(p<0.05).Conclusion: Disintergrin kistrin could induce MMP-2mRNA decreased and did not affect the expression of TIMP-2mRNA . |
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