The Effects Of Rats Islet Cells Functions After Through Vitrification Cryopreservation Pretreatment

Objective:To explore The effects of rats islet cells functions after through vitrification cryopreservation pretreatmentMethods: To separation and purification islet cells by Enzyme digestiong and density gradient centrifugation . Divide the islet cells into two groups at random, Groupâ… were given vitrification frozen pretreatment, Groupâ…¡were given usual pretreatment. Respectively made mould successful transplantation into Balb/c diabetes mice, divided into A group (group), vitrification pretreatment group B (regular training group), and the establishment of group c (diabetes control group). Determination of glucose, weight exp1 mice, and observe exp1 islet graft survival time..Results: A, B group (A = glucose changes between 1.32 mmol/l is averaging 9.8 + B = 11.0, mmol/l) plus or minus 1.17 no obvious difference, not statistically significant, and group C (C = 20.8 mmol/l) plus or minus 1.36 compared to lower blood glucose, A statistically significant; Graft survival time compared group A (12.8 + 2.4 d) than group B (10.6 + 1.8 d), with A statistical significance.Conclusion: Pretreatment group and did not handle group of mice, blood glucose changes between groups differ not quite, pretreatment group islet cells live longer than did not handle group. These show the vitrification pretreatment of islet cells to islet function has no obvious effect even better than conventional training group islet cells. key words:islet cells ,vitrification cryopreservation...