Role Of Endoplasmic Reticulum Stress In SHG-44 Cell Apoptosis Induced By Proteasome Inhibitor MG-132

Objective:To foster the establishment of oxidative stress in H9c2 myocardial cell model, at the same time to select and choose ERK1/2 way as the object of study, the use of ERK1/2 pathway-specific inhibitor UO126 to further explore the possible mechanisms of action, observation of resveratrol on myocardial cells Anti-oxidative stress injury and its mechanism.Methods:The passage ways to nurture myocardial cells. Rats were divided into the following five groups:normal control group, hydrogen peroxide (H2O2) group, resveratrol+H2O2 group, resveratrol+UO126+H2O2 group, UO126+H2O2 group, after the end of the experiment, in an inverted phase contrast microscope observed under the myocardial cells to changes in the measured lactate dehydrogenase (LDH) release, measured superoxide dismutase (SOD) activity, by flow cytometry to detect apoptosis of myocardial cells. Western-blot with the determination of ERK1/2 expression.Results:[1] H2O2 group of LDH significantly increased activity than the control group (998.08±23.07 vs 477.11±17.78, P＜0.05),MDA activity also increased significantly (32.05±1.76 vs 7.42±1.51, P＜0.05),SOD significantly reduced (15.43±4.52 vs 35.03±2.17, P＜0.05) Two groups of comparisons are statistically significant differences.[2] Resveratrol+H2O2 group relatively H2O2 group, LDH active significantly reduced (617.3±11.23 vs 998.08±23.07, P＜0.05), MDA activity also increased significantly(16.33±1.68 vs 32.05±1.76, P＜0.05), SOD activity increased significantly (29.03±3.18 vs 15.43±4.52, P＜0.05) Compared between the two groups are statistically significant differences.[3] Apoptosis rate shows:The cells grow well in the control group,whose apoptosis rate is extremely low at 6.73±2.38. Lots of apoptotic cells in the H2O2 group, whose apoptosis rate is 56.2±4.73 Compared between the two groups are statistically significant differences. (P＜0.05)[4] UO126+H2O2 group than in the control group LDH active significantly reduced (658.19±21.38 vs 998.08±23.07, P＜0.05), MDA activity also significantly reduced (20.01±3.16 vs 32.05±1.76, P＜0.05), SOD activity increased significantly (22.33±1.78 vs 15.43±4.52, P＜0.05). There are statistically significant differences between the two groups. Resveratrol+UO126+H2O2 group and UO126+H2O2 group of LDH, MDA and SOD activity in comparison, there are no significant differences.[5] H2O2 can significantly raise ERK1/2 expression, Resveratrol+H2O2 group can significantly lowered ERK1/2 expression,the difference was statistically significant, there are no significant differences in ERK1/2 expression between resveratrol+UO126+H2O2 group and resveratrol+H2O2 group.Conclusion:1. H2O2 could cause H9c2 myocardial cells to oxidation and apoptosis;2. Resveratrol antagonizes H9c2 myocardial cells to oxidation and apoptosis caused by H2O2, and protect H9c2 myocardial cells;3. Resveratrol plays on the protective effects of myocardial cells H9c2, may be realized through the ERK1/2 channe.