| Objective:To modify bilateral dCST and bilateral RST accurate injury models of cervical spinal cordin rats. Then the expression of caspr and kv1.2 changes to illustrate the two tracts aredifferent responses to injury.Materials and Methods:Self-made blade was used to modify and establish models of bilateral dCST and RSTaccurate injury of cervical spinal cord. The injured spinal cords were taken for observation ofthe morphological changes by HE staining, and neuronal tract tracing by Biotin DextranAmine (BDA) anterograde neuronal tracing technique. Thirty-nine SD rats were dividedrandomly into three groups: bilateral dCST injury group (n=13), bilateral RST injury group(n=13)and sham operation group (n=13). Injury models were established respectively. Threegroups of rats were sacrificed and injury spinal cord specimens were procured at 24h aftersurgery. After the modeling, 2 week and 3 weeks of injury were drawn at the spinal cord byimmunohistochemistry to observe the loss of two tracts of differences in response.Result:As compared with sham group respectively at the time of 1W 3W after injury, expression ofkv1.2 and caspr in all of the injury groups showed differences. Precise location of cervicalspinal cord injury in the two tracts after 2 week and 3 weeks ,compared with the normalgroup, csapr and kv1.2 expression increased, while the increase above the dCST RST theincrease, there is significant difference between the two.Conclusion:Caspr and kv1.2 expression differences were detected between RST and dCST whoseevolution degrees were different at the time of 1W and 3W after injury.The expression ofCaspr and kv1.2 were higher in RST than in dCST after injury. This may indicate thatdifferent expressed in RST which were less evolved were better for protecting injured neuronal tract than genes expressed in dCST. The expression differences may have differenteffects on recovery of the injured neuronal tract and motor function, and deserves furtherresearch. |
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