The Influence Of Different Concentrations Of Salmon Calcitonin Act On The Expression Of IGF-I And The Proliferation Of Suckling Mice Osteoblasts

Objectives:Through observe the role of salmon calcitonin (CT) on osteoblasts in vitro culture rat, we are trying to have a preliminary study of the influence of salmon calcitonin on the proliferation of osteoblasts and the expression of IGF-I, this way could provide a theoretical basis of promoting bone repair and regeneration on clinical treatment.Methods:Take the calvarias of 24h SD neonatal rats, with enzyme digestion of osteoblast cells, primary osteoblasts are obtained by enzymatic digestion, cultured and identified osteoblasts, with different concentrations of calcitonin (1×10 - 12～1×10 - 8 mol/ L) on the osteoblasts cultured in vitro. observed the proliferation of OB by MTT method (MTT staining); Detection of Alkaline phosphatase(ALP) expression by Gomori Calcium - Drilling Method;Observed the Insulin-like growth factor-I (IGF-I) mRNA expression by RT-PCR method. Results: Cells were identified and confirmed by the osteoblasts. MTT assay showed that OB proliferation can stimulate at the concentration of 1×10 - 12 mol / L, and with the increased proliferative capacity of CT concentrations increase in a dose-dependent; Gomori calcium - drill assay results show that with the calcitonin concentration increased ,the ability of the differentiation of OB is enhanced; Detected by RT-PCR showed that IGF-I mRNA in the neonatal rat stable expression in osteoblasts, Semi-quantitative analysis showed that with increasing concentration of calcitonin, IGF-ImRNA expression gradually increased.Conclusion: Appropriate concentration of CT can promote osteoblast cell proliferation; part of the mechanism may be related to CTcould promote the expression of IGF-ImRNA increased.