| Objectives: Searching for the main environmental risk factors of esophageal carcinoma (EC)in changzhi area, Shanxi Province. To study the association between polymorphisms of XRCC1rs915927(Pro206Pro) and rs3547(Gln632Gln) and susceptibility to esophageal carcinoma, and toexplore the interactions of genetic and environmental factors in EC, so as to provide theoreticalevidences for revealing etiological mechanisms of esophageal carcinoma and making theintegrated preventive measures in the high accident place.Methods: 151 patients with esophageal carcinoma and 165 were enrolled in the study. Thecases and controls were matched according by sex and age(±5y), collected from Heping Hospitaland Heji Hospital of Changzhi from Aug 2009 to Jul 2010. The cases were correctly diagnosedby gastro scope and pathology means, and did not have any else tumor. The controls had notumor history and relative disease of esophagus in the same stage. All the cases and controlswere investigated by self-designed questionnaires that contained the related factors of digestsystemic disease. About 3ml venous blood of each person was collected for extracting genomeDNA. So as to detect the genotypes of XRCC1 rs915927(Pro206Pro) and rs3547(Gln632Gln)through polymerase chain-restriction fragment length polymorphism (PCR-RFLP). All the datawere analyzed by SPSS15.0 statistical software. The risk factors of EC were analyzed by noconditional Logistic regression t-test andχ2-test.Results: 1 By non conditional Logistic regression analysis, it was indicated that ten factorswere correlated with EC(P﹤0.05) in the levelα=0.05. By multi-variables non conditionalLogistic regression analysis, it was showed that: liking to eat hard diet and pickled food, eatheat ,fierce mind stimulation were the main risk factors to EC in such area, OR(95%CI) were1.78(1.30～3.65),3.45(1.84～6.50),2.01(1.16～3.48),3.45(1.84～6.50);respectively. Higheducation level was the protective factor to EC, OR(95%CI) was0.55(0.42～0.71)).χ2=6.12,P﹤0.01)2 There wasn't significant association between the genotype distribution of XRCC1rs915927(Gln632Gln) and age, sex, smoking, eating pickled food. XRCC1 rs915927(Pro206Pro)gene have three kinds of genotype: A/A, A/G, G/G; frequencies of them were 92.30%,6.30%,1.40% in case group, 83.60%,15.20%,1.20%,in control group respectively.The difference was remarkable between two groupsχ2=6.12,P﹤0.01. Allele frequencies of Aand G were respectively 95.50%,4.50% in case group, 91.20%,8.80% in control group. Thedifference was remarkable between two groups(χ2=4.34,P﹤0.01).The genotype frequencies ofXRCC1 rs3547(Gln632Gln) were 73.3%,24,2%,2.5% in case group and 74.2%,23.8%,2.0%,in control group respectively(χ2=0.03,P﹥0.05); Allele frequencies of GG and GA wererespectively 79.4%,20.6% in case group, 83.3%,16.7% in control group(χ2=2.68,P﹥0.05).The discrepancy was not remarkable.3 Interaction analytical results: It was showed that there was negative interaction betweeneating pickled food and XRCC1 rs915927(Pro206Pro) mutation gene G on the risk ofEC(attributable proportions of interaction,(API=-0.27 the synergy index S,(S)=0.25). There wasnegative interaction between eating heat and XRCC1 rs915927(Pro206Pro) mutation gene G on the riskof EC(API=-0.27, S=0.24).No interaction between XRCC1 Pro206Pro and XRCC1rs3527(Gln632Gln) was found.ConclusiConclusion: eating quickly,eating hot food,fierce mind stimulation,family history ofcarcinoma were the main risk factors to EC. It was suggested that XRCC1 Pro206Pro notXRCC1Gln632Gln may be concerned with esophageal carcinoma. Caring XRCC1 Pro206Promutation gene G and eating pickled food holds negative interaction。...
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