Alkaloids From Aconitum Kirinense

Aconitum kirinense var. australe W. T. Wang, is a flowering plant blong to Aconitum Linnaeus (Ranunlaceae). This plant is a perennial herbaceous plant, widely spread in Henan Province. It is with strongly toxicity that performs well blood and stopping pains activity and used as a natural pesticide.This paper based on the research of the alkaloids chemical components, Phamacological effect content Determination, abstraction and separating and their application:1.Nine diterpenoid alkaloids have been isolated from the roots of Aconitum kirinense var. australe W. T. Wang. The structures were identified by spectroscopic methods including IR, EIMS, HRMS,1HNMR,13CNMR, DEPT, HMQC, HMBC. The identified compounds are:Delcosine(1), Tuguaconitine (2), Denudatine (3), 14-dehydrobrowniine(4), Lepenine (5), 11a-hydroxy lepenine (6), JiYuan-Aconitine (7), Dehydrolucidusculine (8),14-dehydrodelcosine (9).The compound 7 is a novel C19-diterpenoid alkaloid which was isolated from this plant for the first time.2.The method of acid-alkali titrations was applied to determine the contents of total alkaloids, the optimum conditions were investigated by orthogonal experiment design. The result shows that the yield of the total alkaloids extracted from Aconitum kirinese is 0.545%.Factors influencing the extracting efficiency is as follow:alcohol concentration>alcohol quantity>extract frequency>extracting time.3.In order to investigate the relationship of structure and activity of this plant, three series of derivative of Declosine,Tuguaconitine and Lannaconitine were obtained. The compound D-1 is a novel C19-diterpenoid alkaloid.4.The antimicrobial activity of the compound 1,2,3 at different concentrations were studied by turbidimetry. The MIC of determination of compounds was obtained against Gram-positive (Staphylococcous aureus)and Gram-negative (Escherichia coli) microorganisms.The result shows that the compound 1-3 from Aconitum kirinense exhibited certain bacteiostasis activity to Staphylococcous aureus and Escherichia coli.The growth of the tumor cells was inhibited which were treated with the compound 1,2,5,6 and 7 for 72 h against PC-3 cells by the MTT assay.