Studies On The Chemical Constituents From Metabolites Of Marine Microorganism Aspergillus Terreus And Petroleum Ether Fraction Of Saussurea Involucrate

Objective:To search for new antitumor active leading compounds from marine microorganism. Method:A marine microorganism, Aspergillus terreus, is cultured and upscaled in artificial seawater medium, the corresponding metabolites are isolated and elucidated by using modern spectroscopy techniques. Result:sixteen compounds were yielded from ethyl acetate extraction of Aspergillus terreus mycelium and five compounds are isolated from ethyl acetate extraction of culturing solution. Conclusion:Compounds 1-4 are steroids, compounds 5～8 are organic acids and esters, compound 9 is one alkaloid, compound 10 is one coumarin, compound 11～13 are new compounds, compound 14 is ceramide, compounds 15～16 are butyrolactones, the latter is one new compound, compound 17 is propenyl cyclic pentanediol, compounds 18～21 are cyclic lipopeptides.Marine microorganism Aspergillus terreus (A8-4) are screened and purified from marine sediments collected at nature reserve region in Guangxi It was identified by molecular biology and colony morphology. Pharmacological tests show that the extract of this microorganism is of good antitumor activity.53 liters of Aspergillus terreus are cultured in artificial seawater medium, the mycelium and culturing solution are departed by filteration after fermention. The mycelia are extracted by ethyl acetate. The culturing water phase solution was also extracted with ethyl acetate. After removal of ethyl acetate fromthe aqueous layer solution, the aqueous layer solution was then separated on XAD resin absorption column. Each fraction is analyzed and further separated with a variety of preparative spectroscopy methods.21 compounds are identifed as follows:compound 1(Herbarulide, 1.7mg),2 (Cyathisterone,0.4mg),3(DankasteroneA,2.3mg), 4(DankasteroneB,31.0mg) are steroids, compound 5(Methyl linoleates, 10-.Omg),6(Linoleate,140.Omg),7(Palmitic acid,21.0mg), 8(trans-14-octadecensaeure,31.0mg) are acids and esters, compound 9 (5-N-acetylardeemin,6.0mg)is alkaloid, compound 10(Methylisocoumarin, 56.Omg) is coumarin, compound 11 (Terretrione A,44.0mg),12(Terretrione B,6.6mg),13(Terretrione C,6.2mg) are novel compounds, compound 14 (Newbouldiamide,40.0mg) is ceramide, compound 15 (Butyrolactone, 130.0mg),16 (7"-hydroxy-8",9"-epoxyl butyrolactone I,5.0mg) are butyrolactone, compound 17 (E-4-(1-propenyl-1-yl)-cyclopenta-1,2-diol, 7.0mg) is propenyl cyclic pentanediol, compound 18(Cyclo(L-Val-L-Pro), 19.8mg),19(Cyclo(L-Phe-L-Pro),17.5mg),20(Cyclo(L-Ile-L-Pro),13.5mg), 21(Cyclo(L-Leu-L-Pro),22.9mg) are cyclic lipopeptides.Objective:Petroleum ether fraction of Saussurea involucrata Kar. et Kir. et are separated by chemical and spectroscopy methods, in order to provide standard samples for establishing evaluation method of plant suspension cell line. Method:Compounds were isolated and purified by column chromatography, and their structures were elucidated by chemical and spectroscopy methods. Result:Eight compounds were isolated and identified asβ-sitosterol (Ⅰ), dotriacontanoic acid (Ⅱ), hexacosan-4-olide (Ⅲ), (+)-11α,13-dihydrodehydrocostuslactone (Ⅳ),8α-acetoxydehydrocostus lactone (Ⅴ), zaluzanin C (Ⅵ),11,13α-dihydrozaluzanin C (Ⅶ), 8α-hydroxy-11β-11,13-dihydrodehydrocostuslactone (Ⅷ). Conclusion: Compounds 2-7 were firstly isolated from Saussurea involucrate...