| Objective:To discover the nerve fibers'distribution and innervation on the New Zealand Rabbits cervical vertebra by using the method of retrograde axonal transport of horseradish peroxidase (HRP), in order to definitude the law of innervation on the surface of dura mater of spinal cord and Lamina of vertebra. To provide experimental basis for clinical therapy and be helpful to evaluate and treat the cervical vertigo.Methods:20 New Zealand Rabbits were divided into 4 groups:5 rabbits for dura mater of spinal cord group and 5 rabbits for control group; 10 rabbits for lamina of vertebra groups,5 rabbits for right sides,5 rabbits for left sides and the other sides as control side respectively. In dura mater group,5μl HRP solution was injected onto the surface of C6 dura mater, and 5μl of 0.9% normal saline (NS) was injected in control group. In lamina of vertebra group 5μl HRP solution was injected onto the surface of C5 vertebra lamina of one side and 5μl of 0.9% NS was injected onto the other side respectively. The rabbits were killed by perfusing through ascending aorta after 48 hours. The superior cervical ganglia (SCG),inferior cervical ganglia (ICG) or stellate ganglia and C3～C8 spinal ganglias (SG) of both sides were removed, treated by frozen section method and processed by TMB method. The HRP labeled cells in sections were observed through optical microscope.Results:The HRP labeled cells were found in C5～C7 spinal ganglias of both sides in dura mater group and the labeled cells were found in C4-C6 spinal ganglias of the experimental side. But the labeled cell was not found in the sympathetic ganglias.Conclusion:The fibers of the surface of the cervical dura mater of spinal cord come from the spinal ganglias of double sides, and the fibers of the surface of the vertebra lamina come from the cervical ganglias of the same side, and the fibers of adjacent spinal ganglias overlap on the surface of dura mater or vertebra lamina.Significance:Cervical vertigo is a common disease and often affects the patient's daily life and work severely. Most of them are treated with conservative method. Its'pathogenesis is not very clear and its'diagnosis criterions are not perfect. There are.not good treatment methods for it. The blocking of epidural space and vertebra lamina is an effective method being accepted extensively by scholar, the method can remit the patient's symptom. Some scholars think this method as differential diagnosis of cervical vertigo and other vertigo. This study confirmed the fibers of the dura mater and the vertebra lamina mainly come from the spinal ganglia and are overlap through the method of retrograde axonal transport of HRP. This can improve the cognition of the cervical vertigo's pathogenesis. We suspect the possible pathogenesis of the cervical vertigo:(1) When the fibers of dura mater or vertebra lamina received stimulation, the abnormal impulse enter into spinal cord through the cervical ganglia and go upward to vestibular nuclei, producing false consciousness, i.e. vertigo. (2) The abnormal impulse pass the synapse to the Preganglionic sympathetic fiber then to the Postganglionic sympathetic fiber, at last cause the vertebral to be spasm. The patient feels vertigo because of the ischemia of basilar artery. (3) The above two mechanism interact with each other to induce the patient's vertigo. But the character of the labeled cells is not known clearly and still needs to be approved. The mechanism of cervical vertigo being caused by the stimulation of the nerve terminal still needs to study deeply. |
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