The Role Of PTTG, BFGF, VEGF-C And VEGFR-3 In The Occurrence And Development Of Human Colorectal Carcinoma And The Relationshi Among Them

ObjectiveTo explore the role of PTTG, bFGF, VEGF-C and VEGFR-3 in the occurrence and development of human colorectal carcinoma by detecting the expression of these factors in normal mucosa, colorectal adenoma and colorectal carcinoma as well as the relationship among factors and the relationship of these factors with the clinical pathological characteristic of colorectal cancer patients such as gender, age, differentiation, depth of invasion, lymph node metastasis,and Duke's stage so as to evaluate the relationship between these factors and prongosis.Method80 cases of colorectal carcinoma (CC), 20 cases of colorectal adenoma (Ad) and 20 cases of colorectal normal mucosa(NM)that were surgically removed and certified by pathology from July, 2008 to March, 2009, from Surgical Oncology of Chengde Medical College affiliated hospital were collected. RT-PCR was used to examine the mRNA of PTTG, immunohistochemical staining was used to examine expression of PTTG, bFGF, VEGF-C, VEFR-3, MVD,LMVD.Results1 The expression of PTTG mRNA, PTTG, bFGF, VEGF-C and VEGFR-3 in normal mucosa, colorectal adenoma and colorectal carcinoma.1.1 The expression of PTTG mRNA in NM, Ad, CCPTTG mRNA in CC was significantly higher than that in Ad (P<0.01, P<0.01) and NM (P<0.01, P<0.01). Also PTTG mRNA in Ad was signifycantly higher than that in NM (P<0.01,P<0.01). 1.2 The expression of PTTG, bFGF, VEGF-C and VEGFR-3 in NM, Ad, CC1.2.1 The expression of PTTG and bFGF in NM, Ad, CCThe expression of PTTG and bFGF in CC were significantly higher than those of in Ad (P<0.01, P<0.01) and NM (P<0.01, P<0.01). Also the expression of the two factors in Ad were significantly higher than those of in NM (P<0.01, P<0.01).1.2.2 The expression of VEGF-C and VEGFR-3 in NM, Ad, CCThe expression of VEGF-C and VEGFR-3 in CC were significantly higher than those of in Ad (P<0.05, P<0.05) and NM (P<0.05, P<0.05). Also the expression of these two factors in Ad were significantly higher than those of in NM (P<0.05, P<0.05).1.2.3 MVD, LMVD in NM, Ad, CCMVD in CC (51.18±12.66) was significantly higher than that in Ad (17. 75±3.60, P<0.05), NM (14.67±3.04, P<0.05). And MVD in Ad was significantly higher than that in NM (P<0.05). LMVD in CC (8.76±2.13) was significantly higher than that in Ad (6.25±1.37, P<0.05) and NN (5.30±1.53, P<0.05).2 The relationship between PTTG mRNA, PTTG, VEGF-C, VEGFR-3, bFGF, MVD, LMVD and clinical pathological characteristic of colorectal carcinoma.2.1 The relationship between PTTG mRNA, PTTG and pathological characteristic of CCThe expression of PTTG mRNA,PTTG were positively correlated with depth of invasion, lymph node metastasis, Duke's stage. The expression of PTTG mRNA, PTTG of infiltration more than musucularis in colorectal carcinoma was higher than not (P<0.05, P<0.05).The positive expression of PTTG of lymph node metastasis group was significantly higher than those without lymph nodemetastasis group (P<0.01, P<0.01).The positive expression of PTTG of Duke's CD stage was significantly higher than Duke's AB stage, (P<0.01, P<0.01).Correlations were not found between PTTG mRNA,PTTG and every other characteristics of CC such as gender, age, differentiation degree.2.2 The relationship between bFGF and clinical pathological characteristic of CCThe expression of bFGF was positively correlated with depth of invasion, lymph node metastasis, Duke's stage (P<0.05, P<0.01, P<0.01). Correlations were not found between bFGF and every other characteristics of CC such as gender, age, differentiation degree. (P>0.05, P>0.05, P>0.05).2.3 The relationship between VEGF-C and pathological characteristic of CCThe expression of VEGF-C was positively correlated with depth of invasion, lymph node metastasis, Duke's stage (P<0.05, P<0.05, P<0.05). Correlations were not found between VEGF-C and every other characteristics of CC such as gender, age, differentiation degree. (P>0.05, P>0.05, P>0.05).2.4 The relationship between VEGFR-3 and pathological characteristic of CCThe positive expression of VEGFR-3 was significantly correlated with depth of invasion, differentiation degree, lymph node metastasis and Duke's stage. (P<0.05, P<0.05, P<0.05, P<0.05). The expression of VEGFR-3 was not correlated with patients gender and age (P>0.05, P>0.05).2.5 The relationship between MVD and pathological characteristic of CCMVD was significantly correlated with depth of invasion,lymph node metastasis and Duke's stage. (P<0.01, P<0.01, P<0.01). There were signifycant differences between low-mucous differentiation group and moderate-high differentiateon group (P<0.05). MVD was not correlated with patients gender and age (P>0.05, P>0.05).2.6 The relationship between LMVD and pathological characteristic of CCLMVD was significantly correlated with depth of invasion, lymph node metastasis and Duke's stage (P<0.01, P<0.01, P<0.01).There were signifycant differences between low-mucous differentiation group and moderate-high differentiateon group (P<0.05). Correlations were not found between LMVD and patients gender,age(P>0.05,P>0.05).3 The relationship among PTTG, bFGF, VEGF-C, VEGFR-3, and MVD LMVD3.1 The relationship between PTTG and bFGF, MVD The positive relationship could be seen between PTTG and bFGF, MVD (r=0.72, r=0.70, P<0.01, P<0.01) and be also seen between bFGF and MVD (r=0.72, P<0.01). 3.2 The relationship between PTTG and VEGF-C, VEGFR-3, LMVDPTTG was significantly correlated with VEGF-C, VEGFR-3, LMVD (r=0.69,r=0.72,r=0.67,P<0.01,P<0.01,P<0.01).VEGFC,VEGFR-3were significantly correlated with LMVD (r=0.69, r=0.74, P<0.01, P<0.01).Result1 The high expression of PTTG, bFGF, VEGF-C and VEGFR-3 may play an important role in the occurrence and development of colorectal cancer.2 PTTG, bFGF, VEGF-C, VEGFR-3, MVD, and LMVD was significantly correlated with depth of invasion,lymph node metastasis and Duke's stage in colorectal carcinoma. So they could be a predictive and prognostic factor of CC.3 There were significantly positive correlation among PTTG and MVD in colorectal carcinoma. Suggesting that PTTG and bFGF were cooperated in development of colorectal cancer and metastasis of blood.4 There were significantly positive correlation among PTTG, VEGF-C, VEGFR-3 and LMVD in colorectal carcinoma.Suggesting that PTTG, VEGF-C, VEGFR-3 may interact with each other to promote the generation of lymphatic vessels, thus contribute to the growth,invasion and lymphatic metastasis of colorectal carcinoma.