The Function And Mechanism Of NudC In Cell Migration

Nuclear distribution gene C (NudC) has been reported to participate in chromosome distribution, cytokinesis, and transport of cytoplasmic dynein. However, NudC may have additional functions according to recent research. A number of proteins with various functions have been found to associate with NudC during previous NudC interactome analysis. Here we found that NudC could link Hsp90 and Filamin A to regulate cell migration, which plays critical roles in development, wound healing, immune response, cancer, and so on. The cell migration was impaired after RNA interference of NudC or overexpression of NudC L279P mutation. Furthermore, NudC could biochemically interact with Filamin A, a scaffold protein with considerable significance in the stabilization of microfilament architecture and cell migration. Moreover, both the L279P mutation of NudC and Hsp90 inhibition led to downregulation of Filamin A. The microfilament skeleton also impaired after RNA interference of NudC. Our data thus suggest a novel function of NudC and a novel mechanism within cell migration. That is, NudC may be involved in cell migration by facilitating the stability of microfilament skeleton following stabilizing Filamin A with Hsp90.