Effect Of Sphk1 On Human Colon Cancer Lovo Cells Proliferation And Invasion

AIM: To investigate the effect of Sphk1 on colon cancer cell proliferation, apoptosis and invasion.METHODS: Human colon cancer Lovo cells were divided into Sphk1 activation group, Sphk1 suppression group and control group. Lovo cells were treated with 100nM Phorbol 12-myristate 13-acetate (PMA) as the Sphk1 activation group, and with 50μM erythro-sphingosine, N, N-Dimethyl (DMS) as Sphk1 suppression group, and cells treated with 0.9% Sodium Chloride as control group. Cell proliferation activity was detected by MTT, cell apoptosis was detected by flow cytometry and cell invasiveness was detected by Transwell boden chamber model. Sphk1, ERK1/2 , p-ERK1/2 and NF-κB p65 protein expression were detected by Western blot.RESULTS: PMA can induce the expression of Sphk1 protein in Lovo cells obviously, promote the cells growth and invasiveness but inhibit the cells apoptosis, accompanied with the upregulating of ERK1/2, p-ERK1/2 and NF-κB p65 protein. On the contrary, DMS inhibit the expression of Sphk1 protein in Lovo cells obviously and the cells growth as well, otherwise promote the cells apoptosis, and associated with the supressing of ERK1/2 protein, p-ERK1/2 protein and NF-κB p65 protein expression(0.28±0.017 VS 0.19±0.031, 1.11±0.19 VS 0.28±0.17, 190.57% VS 9.65%, 9.15% VS 32.58%, 0.64±0.054 VS 0.42±0.035, 0.69±0.076 VS 0.37±0.018, 0.29±0.019 VS 0.17±0.026).CONCLUSION: Sphk1 can promote the proliferation and invasion of Lovo cells and inhibit them apoptosis, the mechanism may be related to the activation of ERK1/2 and NF-κB signaling pathways.