| Objective: To detect the protein expressions of hfg12 (human fibrinogen like protein 2) from peripheral blood monocytes as well as liver tissues in patients with different degrees of chronic hepatitis B and liver cancer and try to make clear the correlation and possible involvement between these diseases and the expression of hFgl2.Research hFgl2 prothrombin enzyme positive expression of mononuclear cells and liver total bilirubin (TBil), alanine amino shift enzyme (ALT), the usable acid amino converting enzyme (AST) to determine the correlation between the degree of necrosis of liver cells, and understand the sensitivity of the reflection hFgl2 prothrombin enzyme expression determination of the clinical significance.Methods:1.Acquisition with complete clinical data of 37 cases with chronic hepatitis B, 20 cases of chronic fulminant hepatitis B (group E) and 21 cases of liver cancer (F) peripheral blood smear as experimental, 20 cases of the healthy check-up peripheral blood smear as control group (A);37 cases of chronic hepatitis B according to the guideline of prevention and treatment for chronic hepatitis B (2000 version) [1] is divided into mild (B), medium (C) or severe (D) three groups, mild in 15 cases, moderate 13 cases, severe 9 cases.2.Collected the above 37 cases of chronic hepatitis B with liver biopsy tissues and 21 cases of liver cancer patients surgical resection of the liver tissue.3.Application immunohistochemistry methods were used in the treatment of chronic hepatitis B group, detect Fgl2 protein expression of chronic severe hepatitis B group, liver group and the normal group peripheral blood monocytes.And using the two-step immunohistochemistry methods detected to Fgl2 protein expression in the collected in the liver tissue.4.The statistical data of expression was analysed by SPSS16.0.Results:1.Fgl2 proteins was detected in six groups of peripheral blood monocytes.Its expression of average gray level respectively was(Group A):142.20±1.29,(Group B):147.43±2.64,(Group C):140.04±2.90,(Group D):135.22±10.36,(Group E):135.11±2.10,(Group F):136.30±11. 70.In MIAS medical analysis image,the average gray level of Fgl2 protein in peripheral blood monocytes, the expression of chronic hepatitis B group and chronic fulminant hepatitis group compared ,differences was statistically significant (P < 0.05).The normal group with the different degree chronic hepatitis B groups Fgl2 protein expression comparative difference was no statistically significant (P > 0.05).2.Fgl2 proteins was detected in four groups of liver tissues.Its expression of average gray level respectively was(Group B):182.38±0.99,(Group C):181.84±2.64,(Group D):174.14±1.39. In MIAS medical analysis image,the average gray level of Fgl2 proteins in the liver tissue expression, chronic hepatitis B moderate group and severe group binary comparison, differences was statistically significant difference (P < 0.05).The expression of Fgl2 proteins in chronic hepatitis B light and moderate group,differences was not statistically significant (P > 0.05).3.In peripheral blood monocytes,the expression of Fgl2 proteins average gray level correlates with the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST).(rs respectively was -0.83 and -0.84 ,P<0.05).This indecates that Fgl2 protein expression with chronic hepatitis B severely group of severity were positively correlated.The average gray level of Fgl2 proteins by inspection TBil correlation was not statistically significant difference (P > 0.05).4.In liver cancer group(F group),the average gray level of Fgl2 proteins was respectively 136.3±11.70 and 177.30±6.09 in peripheral blood monocytes and tissue.In peripheral blood monocytes in F group,the average gray level of Fgl2 proteins was different of from A group to E group,differences was statistically significant difference (P< 0.05).But in liver tissue, the average gray level of Fgl2 proteins F group was similiar to the chronic hepatitis B severe groups group(D group).Conclusions:hFgl2 protein can be in the human peripheral blood monocytes and in the liver tissue of in Chronic hepatitis B severe group and liver cancer group.hFgl2 protein expression could be high with the severity degree of chronic liver disease.The expression of hFgl2 prothrombin enzyme in liver cancer group was higher than chronic hepatitis B groups in PBMC.About liver tissue,the expression of Fgl2 prothrombin enzyme was similiar to the chronic hepatitis B severe group.hFgl2 protein expression in organizations and monocytes presented non-synchronous.hFgl2 prothrombin may become monitoring indicators of the liver cell damage degree in Chronic hepatitis B severe group. |
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