Effects Of Aerobic Exercises Pre-intervention On Liver Function ,MDA,IL-10 And Other Indicators In Mice Fed Alcohol

Objective:Aerobic exercise improve the body against free radicals through the antioxidant capacity, lower body resting levels of inflammatory factors enhance the body immunity to improve the collective health of the body; and alcoholic liver injury through lipid peroxidation and inflammation secondary to combat implementation. By ethanol feeding of aerobic exercise on liver function, MDA, IL-10 indices for the aerobic exercise in the prevention of alcohol injury and provide experimental evidence of the phenomenon. Appropriate exercise intensity for the antioxidant, anti-inflammatory capabilities enhance the body, to provide more effective health promotion theory and practical basis.Methods:8-week-old C57BL / 6 mice 60 were randomly divided into 4 groups: normal control group (N), aerobic exercise group (E), aerobic exercise plus ethanol feeding group (E + AL), alcohol irrigation gastric group (AL), build aerobic exercise and alcohol damage the immune model. Where (N) group is neither movement nor stomach, (E) group and (E + AL) group of moderate-intensity exercise for 6 weeks until 6 weeks after exercise (E + AL) group and (AL) group began to ethanol feeding 4 weeks, during (E) group and (E + AL) group to continue to exercise. 10 weeks after the experiment were sacrificed mice in each group: serum, liver, spleen, -20℃preservation. Detection of AST, ALT and other liver function, liver homogenate measured MDA, T-AOC and other antioxidant index, liver biopsy to be prepared by light microscope histological changes. Preparation of spleen lymphocyte suspension system, RT-PCR detection of gene expression of IL-2 detected by flow cytometry the expression of IL-10, measured by ELISA serum levels IL-6 concentration.result:Serum liver function:Compared with N, AL mice ALT, AST levels increased, there was a significant difference (P <0.01); E + AL mice ALT, AST levels increased significantly difference (P <0 .05); E group were ALT, AST content difference was not significant (P> 0.05); and the AL group, E + AL group ALT, AST levels increased, with significant differences (P <0 .05). AL group compared with the N and E + AL group showed pathological changes, indicating successful model. Liver MDA, SOD, T-AOC activity: Compared with N, AL mice MDA content increased, there was a significant difference (P <0.01); E + AL mice MDA content increased, the difference was not significant (P> 0.05) ; E group were lower MDA content, the difference was not significant (P> 0.05); and the AL group, E + AL group with higher levels of MDA, with significant differences (P <0.05). N quiet compared with the control group, AL SOD mice fed alcohol decreased significantly (P <0.01), E + AL aerobic exercise intervention group were also reduced SOD levels, the difference was not significant; but not with alcohol irrigation AL stomach of mice compared E + AL mice aerobic exercise intervention group was significantly higher SOD; significant difference, (P <0.05). Compared with N, AL mice were significantly lower T-AOC, significant differences (P <0.01), E + AL mice increased T-AOC levels, significant differences (P <0. 05), E mice TAOC was significantly increased, the difference was significant (P <0.01); and the AL group, E + AL group of T-AOC was significantly increased, the difference was significantly (P <0 .05).Cytokine expression:The total spleen lymphocytes the expression of IL-10: Compared with N, AL IL-10 spleen cells were significantly increased, the difference was significant (P <0.01), E + AL group of IL-10 Content increased, but the difference was not significant (P> 0.05), E group of lymphocyte IL-10 levels are low, the difference was not significant (P> 0.05). Compared with the AL, E + AL group IL-10 levels were increased, the difference was not significant (P> 0.05). Compared with N, AL, and E + AL IL-6 in serum were significantly increased, the difference was significant (P <0.01), compared with the AL, E + AL IL-6 serum levels in the lower , The difference was not significant (P> 0.05).Conclusion:1. Ethanol feeding caused liver lipid peroxidation, while the immune inflammatory response.2. Simple aerobic exercise intervention to improve antioxidant capacity in mice, aerobic exercise intervention on the ethanol feeding pre-Lipid peroxidation was significantly inhibited; aerobic exercise intervention in pre-ethanol feeding significantly reduced the inflammatory response in mice, of ethanol feeding injury caused by inflammation in rats.3. Effect of exercise on ethanol feeding induced liver injury in rats, possibly through aerobic exercise improve the body's antioxidant capacity, and enhance anti-inflammatory capacity of the body to achieve. This aerobic exercise reduces levels of inflammatory factors, anti-inflammatory effect mechanism may be mediated by IL-6.4. Aerobic exercise intervention group of mice fed alcohol liver damage is still evident, movement only to a certain extent, the scope of prevention and treatment of alcoholic liver injury.