In Vitro Evaluation Of The Microstructural Vascular Scaffold With Micro-Tip Direct Writing Technology

Objective: To evaluate mechanical properties and biocompatibilities of the vascular scaffoldmanufactured with Micro-Tip Direct Writing technology.Methods: Mechanical properties of the vascular scaffold were measured by radial compliance,suture strength and bursting pressure separately. The blood compatibilities of the scaffold wereanalyzed by hemolytic rate test, in vitro dynamic blood clotting test, ant platelet adhesionexperiment. Cell compatibility was evaluated by MTT measurement and morphologicalobservation.Results: The scaffold showed radial compliance of4.03±0.56%/100mmHg, burst strength of102±8KPa, and suture retention strength of 204.5±72.1N/cm2. The hemolytic rate of the scaffoldwas 1.75% .It is lower than 5％which was ruled by ISO. The anti-coagulant property of thescaffolds was significantly better than that of glass slides in dynamic coagulation tests(P＜0.05).The anti-coagulant property of the scaffold was also confirmed by less platelet adhesion. MTTassay results showed that the cytotoxic grade of the novel polyurethane was 0~1. Cellmorphology observation showed that the L929 cells were spindle-shaped or triangular with goodstretch. Conclusion: The scaffold manufactured with the new technique processes goodmechanical properties, blood compatibility and cell compatibility.