| Subject: To use proteomic methods to find the symptoms of chronic hepatitis B Chinese patients between the differentially expressed proteins in plasma were analyzed with chronic hepatitis B Chinese Hou correspondence between the various cards, initially explored the various symptoms of chronic hepatitis B between the Chinese protein group study law, for the clinical syndrome of chronic hepatitis B in Chinese medicine to provide objective indicators for reference and, ultimately, to further explore the symptoms of chronic hepatitis B the molecular basis of traditional Chinese medicine to provide clues. Methods: According to "viral syndrome Chinese standard", in Luzhou Medical College Hospital of Chinese Medicine in May 2008 during the period December 2010 ~~~~~~ inpatient and outpatient chronic hepatitis B patients, the typical hot and humid in the collection retention syndrome (â… ), liver spleen deficiency syndrome (â…¡), liver and kidney Card (â…¢), blood stasis card (â…£), spleen deficiency syndrome (â…¤) and 86 healthy controls were selected as research objects, the first take five cases of each syndrome samples, their plasma protein two-dimensional gel electrophoresis, cut the corresponding differentially expressed protein spots for mass spectrometry analysis, search SWISS-PROT database, and identified differentially expressed more than 2 times the protein, a total of 10 species. Whichever is the more obvious differences in the expression of the syndrome in the regular changes between the five proteins and the expansion of sample size, each card type with 20 cases of normal control group were 5 cases, application of ELISA technique and the normal control group The symptoms of chronic hepatitis B group differences in the protein were detected, and further confirmed in the normal control group and TCM protein expression of the symptom differences between groups. Results: (1) to obtain good resolution and reproducibility than patients with chronic hepatitis B liver depression and spleen group, hot and humid in the resistance group, blood stasis group, liver and kidney group, spleen and kidney yang group and healthy controls The plasma proteome two-dimensional electrophoresis silver staining. (2) mass spectrometry analysis of differential protein spots were identified differential expression of more than 2 times the protein spots 10, which related to immune function, lipid metabolism, inflammation, etc. (3) by ELISA method in which retinol-binding protein (RBP), glass with protein (VN) Haptoglobin (HPT) and immunoglobulin J chain were detected in patients with chronic hepatitis B found that retinol binding protein (RBP), glass with protein (VN), haptoglobin (HPT) lower than the normal control group, by reduction ranges from high to low sort, retinol binding protein (RBP):â…£>â…¢>â…¤>â…¡>â… , glass with protein (VN):â…£>â…¢>â…¤>â… >â…¡Haptoglobin (HPT) has decreased significantly in each group, but no group differences. (4), immunoglobulin J chain syndrome group homogeneous high for the normal control group, the magnitude of increase were:â…£>â…¢>â…¤>â…¡>â… . Conclusion: 1. Initially established chronic hepatitis B liver and kidney cards, spleen deficiency syndrome, hot and humid in the retention syndrome, the liver and spleen deficiency and blood stasis permits the control group plasma proteome 2-DE map. 2. Syndromes of chronic hepatitis B and five multiple protein expression differences between normal, indicating that there are multiple abnormal expression of CHB. Spleen deficiency with chronic hepatitis B, liver and kidney, damp heat resistance, liver spleen, and blood stasis permits multiple differentially expressed proteins, suggesting that the same disease with different cards with different material basis (different protein), this is Chinese "card" the essence of the scientific basis. 3. Syndromes of chronic hepatitis B and five kinds of normal human immunoglobulin J chain expression of significant differences between groups, with statistical significance (P <0.05), which increases blood stasis syndrome group most obvious, suggesting that patients with chronic hepatitis B there is enhanced humoral immune function, and immune dysfunction among different syndromes in varying degrees, to blood stasis syndrome group the most significant immune disorders. 4. Five syndromes of chronic hepatitis B between the different degrees of liver injury to blood stasis permits the most serious liver damage, followed by liver and kidney card, liver spleen deficiency and liver heat retention syndrome in the relatively light damage, with the degree of immune dysfunction syndrome group is consistent... |
PDF Full Text Request