The Expression Of KLK 10 In Endometrioid Adenocarcinoma And Its Correlation With ER, PR

The expression of KLK10 in endometrioid adenocarcinoma and its correlation with ER, PRObective:This study was designed to evaluate the expression of KLK10 in normal endometria , endometrial hyperplasia, endometrioid adenocarcinoma and to investigate the relationships between their expressions and the clinicopathological parameters and prognosis respectively, and we also analysed the correlation of KLK10 expressions with ER, PR in endometrioid adenocarcinoma, which may reveal the role of KLK10 in the pathogenesis and progression of endometrioid adenocarcinoma and the correlation with ER, PR. Thus we would find promising targets for diagnosis and therapy.Method:The research objects were 19 endometrial hyperplasia tissues and 34 endometrioid adenocarcinoma tissues, the control group was 12 normal endometrial. Immuno- histochemical methods were used to detect the protein expression of KLK10 in different tissues. To compare the difference of the expression in different tissues and to analyse the expression of KLK10 in endometrioid adenocarcinoma and the relationship with clinical parameters, such as surgical pathology staging, histopa-thological grading, and age. We also detected ER, PR expression in 34 cases of endometrial adenocarcinoma and analyzed the correlation between them. Preliminary discussed the relations of KLK10, ER and PR in endometrial adenocarcinoma occurrence and development. The correlation of the expression of KLK10 in endometrial adenocarcinoma, normal endometrial tissue and endometrial hyperplasia, the correlation of the factors with the clinical features and the correlations of KLK10, ER and PR expression levels in adenocarcinoma endometrium were evaluated by chi-square test, fisher probability method and the Pearson correlation analysis.Results: 1. KLK10 protein immunoreactivity was observed in the cytoplasm. Positive KLK10 protein immunostaining were observed in 13 of 34(38.2%) endometrial carcinoma samples, 11 of 12(91.7%) normal endometria, 15 of 18(78.9%) endometrial hyperplasia, average gray values were 151.09±21.54, 125.25±21.47, 135.08±30.67, average gray values were 151.09±21.54,125.25±21.47,135.08±30.67. The expression of KLK10 in endometrial carcinoma was significantly less than that in normal endometrial hyperplasia (P<0.05). The positive expression rate in Surgical pathology stageⅠ,ⅡandⅢwere 50.0%(3/6), 50.0%(3/6), 31.8%(7/22),average gray values were 148.47±30.51, 143.63±35.77, 168.16±23.94, the positive expression rate in histological grade 1, 2 and 3 were 36.3%(4/11), 41.2%(7/17), 33.3%(2/6), average gray values were 151.43±29.45, 150.97±33.51, 150.80±29.67, the positive expression rate in be equal or greater than 50 and over 50 were 33.3%(5/15), 41.2%(8/19), average gray values were 161.45±29.19 ,142.91±30.03. There were no significant association between KLK10 expression and Surgical pathology stage, histological grade and age.2. Positive ER immunostaining were observed in 23 of 34(67.6%) endometrial carcinoma samples. Positive PR immunostaining were observed in 19 of 34(55.9%) endometrial carcinoma samples. The positive rates of KLK10 expression were 45.5% (5/11) in ER-negative carcinomas, 69.6% (16/23) in ER-positive carcinomas. The positive rates of KLK10 expression were 33.3% (5/15) in PR-negative carcinomas, 78.9% (15/19) in PR-positive carcinomas. The expression of KLK10 was positively correlated with ER expression (P<0.01) and PR expression (P<0.01).Conclusion:1. The positive expression rate of KLK10 in endometrioid adenocarcinoma was significantly higher than normal endometrial and endometrial hyperplasia tissue; the expression of KLK10 had no relation with the age, histological classification and clincal stage. This suggested that absence or downregulated expression of KLK10 possibly played an improtant role in the genesis and development of endometrioid adenocarcinoma. 2. The expression of KLK10 in endometrioid adenocarcinoma was lessen, The expression of ER, PR in endometrioid adenocarcinoma was lessen. KLK10 protein in endometrial adenocarcinoma tissues and ER, PR in endometrial adenocarcinoma tissues have a certain correlation. We speculated that KLK10 gene might be involved in ER, PR expression regulation. KLK10 protein alone or with ER, PR interaction among affect endometrial growth and apoptosis.