Effect Of Specific Antagonism CXCR4 By AMD3100 On Bladder Carcinoma Cell Intraluminal Implantation

Objective:To research the effet of the antagonist AMD3100 to the proliferation, invasion, migration, oncogenic rate and intraluminal implantation of EJ-m3 which are human bladder cancer cell subsets with high invasiveness.Methods:With EJ-M3 as research model, useing AMD3100 of different concentrations acting on EJ-M3 cell,and then detecting the changes about cell growth curve with cell count board.Detecting the changes of cell proliferation after different concentration AMD3100 acting on EJ-M3 cell by MTT method.Determined the cell invasion with Millicell chamber method which was based on penetrating artificial basement membrane and polycarbonate membrane of 8um pore sizeafter different concentration AMD3100 acting on EJ-m3 cell 24 hours. Detected the changes of migration after different concentration AMD3100 acting on EJ-m3 cell through the scratch test;observed the oncogenic rate situation of nude mice intravesical planting about experimental group and control group after AMD3100 acting on EJ-m3 cell.Results:In-vitro,it was founded that the growth curve of experimental group significantly lowering to the control group and showing concentration-depended by detecting the changes about cell growth curve after different concentration AMD3100 acting on EJ-m3 cell.It was showed that the proliferation rate of experimental group clearly droping to the control group and showing concentration-depended through MTT method.The result of Millicell Chamber invasion experiment indicated that the number of cell transmembrane decreased with the increasing concentration of AMD3100 that acting on the EJ-m3 cell 24 hours(P<0.05).Detected the different about migration between the EJ-M3 group that was from bladder tumor cells with high inasive and the EJ-M3 group that was from applicating the antagonist AMD3100 of CXCR4 by the scratch test,it was found that the migration of the latter decreased significantly at 18 and 24 hour to the former and showed concentration-depended (P<0.05). In vivo,we successfully constructed the model of bladder tumor cavity planting,and applyed the antagonist AMD3100 of CXCR4。The result was that the oncogenic rate of AMD3100 group less than the control group (P=0.019)Conclusion:It was found that the appreciation, invasion and migration of experimental group less than control group significantly and showing a concentration dependent by successfully applying MTT method, invasion assay and scratch test detecting biological characteristics of the EJ-M3 group that was from bladder tumor cells with high inasive and the EJ-M3 group that was from applicating the antagonist AMD3100 of CXCR4 in vitro.Further more, the oncogenic rate of experimental group less than the control group clearly by Constructing bladder tumor model and observing in vivo.