Experimental Study On Athletic Secondary Amenorrhea Regulated By Acupuncture And Moxibustion

ObjectiveThis experiment was established to set up a rat model of long-term steadily increasing training exercise which was similar with the athletics. Its purpose was to investigate how the training control rat's estrogen cycle, what were the changes of relevant organs and how the training affected the reproductive endocrine, the HPOU Axis and HPA Axis.So it could understand the generation mechanism of Athletic Menstrual Cycle Irregularities and Athletic Secondary Amenorrhea. Then it could inference that how training affected female menstruation, female HPOU Axis and HPA Axis. At the same time, it could reaserch whether acupuncture and moxibustion could regulate Athletic Menstrual Cycle Irregularities and Athletic Secondary Amenorrhea. What was the mechanism of Acupuncture and moxibustion. It could provide new ideas and methods that prevent Athletic Menstrual Cycle Irregularities and Athletic Secondary Amenorrhea. At the same time, the experiment could find out the best way could play a better role in the sports training by comparing different acupuncture methods.MethodsThere were 45 16-weeks-old clean healthy female SD rats that were randomly divided into 5 groups, including normal control group, steadily increasing training group, steadily increasing training with electric Acupuncture group, steadily increasing training with Moxibustion group and steadily increasing training with electric Acupuncture and Moxibustion group. There were 9 rats in each group that set up Athletic Secondary Amenorrhea rat model after long-term steadily increasing training exercise. Then the rats were cured with Acupuncture and Moxibustion. After experiment we could observe exfoliated cells from vaginal. We could detect the levels of E2,P,FSH,LH,T,Cort by radiation immune method. We also detected proportion of uterine, ovarian, pituitary and heart. We could observe how the exercise affected rat's estrogen cycle, what were the changes of relevant organs and how the training effected the reproductive endocrine, the "Hypothalamus-Pituitary-Ovarian-Uterine Axis" after Acupuncture and Moxibustion. And we could know the relationships from each other.Results1. Vaginal exfoliated cells examinationBy observing vaginal exfoliated cells of the rat from microscope every day, we could see that the rats from each group have relatively stable Estrogen period before training. After seven weeks training, we could observe vaginal smear from steadily increasing training group that the number of nuclear op(?)helial cells and keratinocyte cells reduce before the Estrogen period During the Estrogen period the keratinocyte cells obviously reduce and we could see few keratinocyte cells. During the Estrogen interphase period, the leucocyte cells and mucous increase. The Estrogen period become indisipline such as Estrogen period is shortened and the Estrogen interphase period is prolonged. And because the vaginal smear is in Estrogen interphase period for a long time, it appears a phenomenon that Estrogen period is inhibited by training.2. Reproductive endocrine examination(1) The E2 levels from steadily increasing training group are significantly lower than normal control group(P<0.05). The E2 levels from steadily increasing training with Moxibustion group are significantly lower than normal control group (P<0.05)(2) The P levels from steadily increasing training with Moxibustion groupand steadily increasing training with electric Acupuncture group are significantly higher than normal control group and steadily increasing training group (P<0.05)(3)The T levels from steadily increasing training group are significantly (?) than normal control group (P<0.05). The T levels from steadily increasing training with electric Acupuncture and Moxibustion group are significantly higher than steadily increasing training group (P<0.05)(4) The FSH levels from steadily increasing training with Moxibustion group,steadily increasing training with electric Acupuncture group and steadily increasing training with electric Acupuncture and Moxibustion group are significantly higher than normal control group (P<0.01). The FSH levels from steadily increasing training with Moxibustion group and steadily increasing training with electric Acupuncture group are significantly higher than steadily increasing training group (P<0.05)(5) The LH levels from steadily increasing training with Moxibustion group, steadily increasing training with electric Acupuncture group and steadily increasing training with electric Acupuncture and Moxibustion group are significantly higher than normal control group (P<0.01). The LH levels from steadily increasing training with Moxibustion group, steadily increasing training with electric Acupuncture group and steadily increasing training with electric Acupuncture and Moxibustion group are significantly higher than steadily increasing training group (P<0.01).3. The visceral proportion from rats(1) The uterine proportion between steadily increasing training with Moxibustion group and steadily increasing training with electric Acupuncture and Moxibustion group is significantly different (P<0.05)(2) The pituitary proportion of steadily increasing training group is significantly higher than normal control group (P<0.05). The pituitary proportion from steadily increasing training with Moxibustion group and steadily increasing training with electric Acupuncture group are significantly lower than steadily increasing training group (P<0.05)(3) The heart proportion of steadily increasing training with Moxibustion group and steadily increasing training with electric Acupuncture and Moxibustion group are significantly higher than normal control group (P<0.05).Conclusion(1) Because steadily increasing training can make levels of E2 and T significantly lower than normal group in the male rat, it shows that the Training can control rat's estrogen cycle and affect the function of ovarian. So steadily increasing training can affect the terminal side of the HPOU axis.(2) The level of E2 significantly increased after the treatment of acupuncture and acupuncture with moxibustion together. Acupuncture with moxibustion together can make the level of E2 close to normal. It makes sure that acupuncture with moxibustion can adjust reproductive hormone.(3) The levels of P,FSH and LH significantly increased after the treatment of acupuncture and moxibustion only. The level of P significantly increased after the treatment of acupuncture with moxibustion together.(4) The treatment of acupuncture is the most effective for the levels of P and FSH. The treatment of moxibustion is he most effective for the level of LH. The treatment of acupuncture with moxibustion together is he most effective for the level of T.(5) Steadily increasing training can make effect from ovarian which is terminal side of the HPOU axis. And it can affect hormone. Acupuncture and moxibustion can adjust the meridian and central nervous,and then adjust the central side of the HPOU axis. It can affect the function of hypothalamus and pituitary which can promote to release GnRH. And because the levels of FSH and LH change, the function of ovarian which is terminal side of the HPOU axis changes together.All these changes can affect the levels of E2 and T.So the effect from steadily increasing training is different from the effect from acupuncture and moxibustion. Acupuncture and moxibustion not only can affect ovarian and pituitary function, but also they can adjust endocrine level. Then acupuncture and moxibustion influence menstrual cramps. That is the mechanism acupuncture and moxibustion can adjust Athletic Secondary Amenorrhea.