The Change And Clinical Significance Of Serum Cytochrome C And SFas In Neonatal Sepsis

Objective We investigate the levels of serum cytochrome c and soluble Fas(sFas) in the newborns with neonatal sepsis to evaluate the relationship between cytochrome c/sFas and neonatal sepsis and to provide significant index that may be reflect the severity of neonatal sepsis.Methods 84 neonates who were admitted to the NICU of the second hospital of Lanzhou University from November in 2009 to September in 2010 were enrolled. According to the severity of infection,the patients were invided into four groups:severe neonatal sepsis group, neonatal sepsis group,mild infection group and non-infection group.Serum samples of neonates were obtained on the first and the third and the sixth hospital day. Serum levels of cytochrome c and soluble Fas were measured by enzyme-linked immunosorbent assay(ELISA).Meawhile,the results of WBC,CRP,PLT,CKMB and AST were performed.Results1. The levels of serum cytochrome c were significantly higher in infants with severe neonatal sepsis group and neonatal sepsis group than those in infants with mild infection group and non-infection group (P<0.01). In the first day on admission,the levels of serum cytochrome c in patients have no statistical difference between those with severe neonatal sepsis group and neonatal sepsis group. In the third and sixth day, the levels of serum cytochrome c increased significantly in the patients with severe neonatal sepsis group than neonatal sepsis group.2. The levels of soluble Fas were significantly higher in infants with severe neonatal sepsis group than those in infants with mild infection group and non-infection group in the third and sixth hospital day (P<0.01). And there was no significant difference between the neonatal sepsis group and mild infection group and non-infection group (P>0.01).3. Pearson correlation analysis showed that serum cytochrome c levels correlated with PLT,AST,CKMB and the levels of soluble Fas correlated with AST,PLT.Conclusions The levels of serum cytochrome c and soluble Fas evidently increased in neonatal sepsis,so we concluded that the disorder of cell apoptosis maybe play an important role in progression of neonatal sepsis. Monitoring serum cytochrome c and sFas levels may be useful to assess the severity and the prognosis of neonatal sepsis.