The Effect Of Sorafenib On Growth, Apoptosis And Expression Of P-ERK Of Human Gastric Cancer SGC-7901 Cell

Objective: To investigate the multiple molecular targeted agents sorafenib in human gastric cancer SGC-7901 cell proliferation, apoptosis and expression of p-ERK, and explore its possible mechanism.Methods: MTT method was used to detect antiproliferative ratio of sorafenib on human gastric cancer SGC-7901 cell; Immunocytochemical detection of gastric cancer cells P-ERK protein expression; flow cytometry to analyze the gastric cancer cell apoptosis.Results: sorafenib could obviously inhibit proliferation of gastric cancer cell and appeared time-dose-dependent effects (P<0.05); When gastric cancer SGC-7901 cells were treated with sorafenib, immunocytochemistry showed that p -ERK expression was significantly decreased (P<0.05); flow cytometry showed that the SGC-7901 cell apoptosis rate increased (P<0.05). Conclusion: Sorafenib can significantly inhibit human gastric cancer SGC-7901 cells growth in vitro, the main mechanism is the inhibition of p-ERK expression, thereby inhibiting their proliferation and promote apoptosis.