| Plasma membrane(PM) is the structural base of all cells. Proteins at the PM allow cells to communicate with each other and with the extracellular environment and are critical for the propagation of signaling cascades, vesicle trafficking, ion transport, and so on. There are two-thirds drug target belongs to membrane proteins. Hence, it is important to study plasma membrane proteomics because of its theoretical significa- nce and application prospect. However, the analysis of membrane proteins has always been hampered by their hydrophobic properties and relatively low abundance.In order to explore the occurrence and development mechanism of gastric cancer, identify oncology markers and develop new therapeutic targets for gastric cancer, fluorescein/biotin double-labeling—avidin enrichment technology is adopted to purify the plasma membrane proteins of gastric cancer. The next, those plasma membrane proteins is separated through SDS-PAGE, following by LC-MS/MS and bioinfor- matics analysis. 89 proteins have a gene ontology(GO) annotation information in 118 non-redundant proteins identify by this way. There are 36 (40.45%) proteins belong to membrane protein, including 25% binding and structural proteins, 19.4% Cell proliferation, differentiation and apoptosis related proteins, 16.7% signaling proteins and receptor,13.9% ion channel and transport protein, 8.3% cell cycle-associated proteins, 8.3% Metabolism-associated proteins and 8.3% other annotated proteins.This results show that our study establish a set of research strategy for plasma membrane proteomics of cancer cells, complement the plasma membrane proteomics databases of gastric cancer, find out R-Cadherins,S100A9,HSP 90,CASK,AIF protein. |
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