The Effect Of Nitric Oxide On The Damage Of Pancreas And Kidney In Rats In Acute Necrotizing Pancreatitis With Hyperlipidemia

Objective: 1.Through building models of hyperlipemia (HL)and hyperlipidemia acute necrotizing pancreatitis(ANP) in rats:1.Observe the effect of endodermis nitric oxide synthetase(eNOS)and nitric oxide(NO) when the TG influence,understand eNOS and the correlation between changes in the level of NO;2.utilize L - arginine (L-Arg) and levorotatory nitro arginine methyl ester (L - NAME) interfere in rats with hyperlipidemia acute necrotizing pancreatitis (HL type of ANP), From both positive and negative aspects discuss the correlation between NO and eNOS, determine the role of nitric oxide(N0) on the damage of pancreas and kidney in rats with hyperlipidemia acute necrotizing pancreatitis and the effect of glomerular podocyte.Merhods: 50 male SD rats were randomly divided into 5 groups: Bal group,HL group,HL+ANP group,HL+ANP+L-Arg group,HL+ANP+L-NAME group.The specific methods are as follows:First, random distinguish 10 as Bal group, and feed on 28 days, the remaining 40 only feed high-fat diet( main ingredients for 87.8 % base diet,10 % lard,2% cholesterol and 0.2 % bile acid sodium salt ) on 28 days to establish HL model rats ; 28 days after 40 HL rats random divided into four groups (n=10),the Bal group and the HL group were used retrograde injection Sodium Chloride, HL+ANP group,HL+ANP+L-Arg group,HL+ANP+L-NAME group were used retrograde injection 3.5% sodium taurocholate to build the model of rats suffered from ANP,HL+ANP+L-Arg group made by the modal 30 minutes before and after 2 hours with abdominal injection L-Arg,each 100mg/kg, HL+ANP+L-NAME group for the same time and method inject L-NAME, each 10mg/kg. all rats were sacrificed after six hours,measured amylase(Amyl),creatinine(Cr),urea nitrogen(BUN) and NO of blood ,NO of pancreas,eNOS,histopathology of pancreas,election microscopy of glomerular podocyte, nephrin in glomerular podocyte were examined by immunohistochemistry.Results: Compared with Bal group , HL group of serum Amyl,Cr,BUN level has increased(P<0.05),NO of serum,and pancreas concentration decreased obviously(P<0.05), pancreatic tissue oedema,inflammation,pathological score increased(P<0.05), eNOS of pancreas and glomerular podocyte nephrin dyeing level is relatively shallow(P<0.05)serum TG increased significantly(P<0.01); compared with HL group,HL+ANP group,HL+ANP+L-Arg group,HL+ANP+L-NAME group of serum Amyl,Cr,BUN level increased obviously(P<0.001-0.05);compared with HL+ANP group,HL+ANP+L-Arg group of serum Amyl,Cr,BUN level has decreased(P<0.001),NO of serum,and pancreas concentration increased obviously(P<0.001-0.01),damage of histopathology of pancreas mitigation (P<0.001-0.05),eNOS of pancreas and glomerular podocyte nephrin dyeing degree is deep(P<0.01-0.001);compared with HL+ANP group,HL+ANP+L-NAME group of serum Amyl,Cr,BUN level has increased obviously(P<0.001),NO of serum, and pancreas concentration decreased(P<0.001-0.01),damage of histopathology of pancreas aggravated(P<0.001-0.05),pancreas eNOS, glomerular podocyte nephrin dyeing degree shallow,coloring cell significantly reduced(P<0.001-0.05).Conclusion:1.An ideal animal model of HL can be builded by feeding high-fat diet for 4 weeks.A model of ANP complicated with hyperlipemia in rat might be developed by feeding high-fat diet for 4 weeks and retrograde injection of 3.5% sodium taurocholate into the pancreatic duct;2.?HL as one of the redused reason of eNOS,direct ratio of the level of eNOS;3.When hyperlipidemia acute necrotizing pancreatitis injured renal dysfunction,NO which comes from eNOS not only protect pancreas ,but also kindey function,to glomerular podocyte also has certain protective effect.