Expression Of GLUT-1,TNF-a And P27 In Non Small Cell Lung Cancer

BACKGROUND AND OBJECTIVEGlucose transporter (GLUT) cells is mediated by the main carrier of glucose uptake,existed in human tissues cells widespread。Recent studies showed that members of the GLUT family of fourteen species, of which GLUT-1 is the most widely distributed of the known in vivo transporter which were in all tissues GLUT-1 overexpression concerned with tumor diseases, hypoxic ischemic encephalopathy, type 2 diabetes and other diseases.Tumor necrosis factor-a ( TNF-α) is an important neurotransmitters of inflammatory response and a series of pathophysiological processes in vivo it is mainly composed of mononuclear macrophages and involved in the immune process.TNF-αis secreted mainly by activated monocytes and macrophages, can directly kill tumor cells,make tumor cell apoptosis.TNF-αis a cytokine with multiple functions, it can act directly on pancreaticβcells, resulted inβ-cell damage, induced insulin resistance.TNF-αcan inhibit insulin signal transduction and other indirect effects of cell or tissue, which leads to insulin resistance. P27 kip1 is also known as cyclin dependent kinase inhibitor (CDKI) family member, is a novel candidate tumor suppressor gene, in vitro Cylin E. cdk2, Cyclin D. cdk4 closely integrated ,the activity of which play depends on the relationship between the three doses of the chemical.P27 is the CKD inhibitory protein, can directly inhibit a variety of CDK expression and activity, and expression of P27's too strong to stop at G1 phase cells, causing increased protein synthesis. High glucose decreased expression of P27 .To investigate the expression and clinical significance of TNF-a, GLUT-1and P27 in human non-small cell lung cancer (NSCLC) tissues under high glucose,and Diabetes mellitus (DM) affects gene expression and clinical significance MATERIAL AND METHODSThe clinical data and 100 cases of patients with lung cancer in our hospital in 2009 were collected, and 24 patients with benign lesions served as benign comparison group. By pre-operation inspection, the patients had no distance metastasis, no operation constrain indication, no anticancer therapy before operation, and the postoperative pathology was confirmed to be non-small cell lung cancer。Useed immunohistochemical method to detect the express of GLUT-1,TNF-αand P27 of 100 cases of non-small cell lung cancer (56 cases of diabetes mellitus)and 24 cases of benign tumor(12 cases of diabetes mellitus)and analysis of clinical and pathological features between three index and the impact of diabetes on gene expression and clinical significanceRESULTS(1)The GLUT-1 and TNF-a protein of NSCLC tissues was significantly higher than that of benign tumor(p﹤0.05)but the P27 protein positive rate was significantly lower than the benign tumor(p﹤0.05)(;2)The protein positive rate of GLUT-1 in DM, NSCLC tissue was significantly higher than the NSCLC group, P27 in DM, NSCLC tissue was significantly lower than the NSCLC group TNF-a had no significant statistical difference.;(3) Benign tumor group:The GLUT-1 expression in positive blood glucose were significantly higher than the negative group(p﹤0.05). P27 expression in positive blood glucose were significantly lower than the negative group(p﹤0.05);(4)GLU T-1 expression: Positive group was significantly higher than blood glucose negative, T3 group were significantly higher than T2, T2 group was higher than T1(p﹤0.05), lymph node metastases was significantly higher than those without lymph node metastases(p﹤0.05), N2 was significantly higher than that of N1 group(p﹤0.05),Ⅲstage of NSCLC was significantly higher than in theⅡstage of NSCLC tissue(p﹤0.05),Ⅱstage was significantly higher than in theⅠstage(p﹤0.05; (5)TNF-a group: lymph node metastases was significantly higher than those without lymph node metastases(p﹤0.05), N2 group was significantly higher than the N1 group(p﹤0.05),Ⅲstage of NSCLC was significantly higher than in theⅡstage of NSCLC tissue(p﹤0.05),Ⅱstage was significantly higher than in theⅠstage(p﹤0.05); (6)P27 group: blood glucose positive group was significantly lower than the negative group, no lymph node metastasis group was higher than with lymph node metastasis(p﹤0.05),Ⅲstage of NSCLC was significantly lower than in theⅡstage of NSCLC tissue(p﹤0.05),Ⅱstage was significantly lower than in theⅠstage (p﹤0.05).CONCLUSION(1)The overexpression of TNF-αand GLUT-1 in NSCLC and low expression of P27 have close relationship with the lung cancer growth, invasion and metastasis. (2) The high expression of GLUT-1 low expression of P27 in Diabetes and lung cancer suggesting that diabetes may be related to invasion of tumor growth.