Quantitative Analysis Of S100 Protein Concentration In The Subretinal Fluid Of Rhegmatogenous Retinal Detachment

Objective Detect the concentration of S100 protein in the subretinal fluid of rhegmatogenous retinal detachment. Analasis S100 protein's differences in age, gender and disease duration of rhegmatogenous retinal detachment. Try to assess the neuronal suffering in rhegmatogenous retinal detachment by detect the concentration of S100 protein in the subretinal fluid.Methods The puncture was performed in the sclera with a 25G needle, and the fluid was collected with a plastic sealed tube. Nineteen subretinal fluid samples were obtained from nineteen eyes with rhegmatogenous retinal detachment undergoing retinal detachment surgery. And take nineteen serum samples from these patients as the experimental group. Then take seven normal serum samples as a control group. Use douboe antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect S100 protein's the concentration of samples. Use Mann-Whitney rank sum test to analysis differences between groups.Result Nineteen subretinal fluid samples'median is 1053.57 ng/L and range is from 989.29 ng/L to 1178.57 ng/L. Nineteen serum samples'median is 275 ng/L and range is from 105 ng/L to 475 ng/L. Seven normal serum samples'median is 285 ng/L and range is from 215 ng/L to 385 ng/L。The difference of S100 protein's concentration in the subretinal fluid,between age,sex,and disease duration,was not statistically significant(P>0.05). S100 protein concentration's difference of these patients who have rhegmatogenous retinal detachment,between in the subretinal fluid and in the serum,was statistically significant(P<0.01).Conclusion S100 protein exists in the subretinal fluid.And this means neuroepithelial suffering should occur in rhegmatogenous retinal detachment. The blood-retinal barrier dose not lose all function in the development process of rhegmatogenous retinal detachment.