Relationship Between The Endoplasmic Reticulum Stress In Placenta Tissues And The Pathogenesis Of Preeclampsia

Objective:To explore the relationship between the endoplasmic reticulum stress in placenta tissues and the pathogenesis of preeclampsia.Methods: Thirty preeclampsia patients (PE group), hospitalized in Department of Obstetrics first affiliated hospital of Nanjing Medical University from July 2009 to January 2010,were selected as the subject group and 35 normal pregnant women as control group. Electron Microscopy was used to measure morphology of the endoplasmic reticulum in these women placenta trophocyte. Western blot assays were performed to determine the expression levels of GRP78/BIP,GRP94,caspase-12,CHOP protein in placenta.Results: (1) The ER within the syncytiotrophoblast was observed to be markedly dilated in PE group compared with control group;(2)The expression levels of GRP78/BIP,GRP94protein in placenta of PE group (0.81±0.31and0.55±0.24) were significantly higher than those in placenta of control group (0.40±0.10 and 0.22±0.09; P<0.01).(3)The expression level of caspase-12,CHOP protein in placenta of PE group (4.03±0.65 and 1.56±0.17) were significantly higher than those in placenta of control group (1.85±0.85 and 0.91±0.69, P<0.01). Conclusions: The obvious expansion of endoplasmic reticulum in trophocyte and the increased expression levels of GRP78,GRP94 and caspase--1 2,CHOP indicate that endoplasmic reticulum stress—mediated apoptosis may be involved in the pathophysiological processes of PE.